Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Plant Regeneration Via Direct Adventitious Roots from Free Root Segments of Ulmus davidiana Planch

당느릅나무(Ulmus davidiana Planch) 부정근 절편으로 부터 부정아 유도를 통한 식물체 생산

  • Kim, Ji-Ah (Division of forest resources, college of forest and environmental sciences, Kangwon National University) ;
  • You, Xiang-Ling (Division of forest resources, college of forest and environmental sciences, Kangwon National University) ;
  • Ahn, Chang-Ho (Division of forest resources, college of forest and environmental sciences, Kangwon National University) ;
  • Lee, Jae-Seon (Division of forest resources, college of forest and environmental sciences, Kangwon National University) ;
  • Choi, Yong-Eui (Division of forest resources, college of forest and environmental sciences, Kangwon National University)
  • 김지아 (강원대학교 산림과학대학 산림자원학부) ;
  • 유향령 (강원대학교 산림과학대학 산림자원학부) ;
  • 안창호 (강원대학교 산림과학대학 산림자원학부) ;
  • 이재선 (강원대학교 산림과학대학 산림자원학부) ;
  • 최용의 (강원대학교 산림과학대학 산림자원학부)
  • Received : 2006.12.28
  • Accepted : 2007.03.07
  • Published : 2007.03.31
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Abstract

Micropropagation of Ulmus davidiana Planch was established via adventitious shoot formation from the segments of adventitious roots. Adventitious roots were produced directly from root segments of seedlings on a 1/2 SH medium plus various concentrations of IBA. The maximum growth of adventitious roots was observed in the presence of 2.0 mg/L IBA. After the segments of adventitious roots were cultured on various cytokinins (zeatin, 2-iP, BA, kinetin) and cytokinins plus auxin (IBA), formation of adventitious shoot was investigated. Among cytokinin treated, kinetin was the most effective on both adventitious shoot induction and number of shoots. Especially, 2.0 mg/L kinetin was the best to increase adventitious shoot induction (95.8%) and a number of shoots (8.4). Adventitious shoots were rooted on 1/2 WPM medium and the plantlets were acclimated 100% on composed soil (peatmoss : vermiculite 1 : 1).

당느릅나무(Ulmus davidiana Planch) 기내번식 방법을 개발하기 위해 유식물의 뿌리로부터 부정근을 유도 한 후 이들 부정근 절편으로부터 부정아 유도과정을 거쳐 식물체를 재생시켰다. 기내발아 식물체의 뿌리 절편을 0.2 mg/L IBA 처리한 1/2 SH 배지에 배양한 결과 다수의 부정근을 유도할 수 있었다. 유도된 부정근으로 부터 절편을 만들어 부정아 유도를 위해 MS배지에 4가지 종류의 cytokinin(zeatin, 2-iP, BA, kinetin)을 첨가한 결과 0.2 mg/L kinetin을 첨가한 배지에서 가장 높은 부정아 유도율과 절편 당 부정아수를 얻었다. 유도된 부정아로부터 줄기생장 및 뿌리유도를 위한 실험에서 줄기 생장은 1/2 WPM배지에서 가장 양호하였고, 뿌리유도는 1/4 WPM배지에는 가장 양호하였다. 또한 기내 재분화묘를 기외로 순환시킨 결과 4주후 100%의 식물체 생존율을 얻었다.

Keywords

Acknowledgement

Supported by : 산림청

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