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Plant Regeneration Via Direct Adventitious Roots from Free Root Segments of Ulmus davidiana Planch  

Kim, Ji-Ah (Division of forest resources, college of forest and environmental sciences, Kangwon National University)
You, Xiang-Ling (Division of forest resources, college of forest and environmental sciences, Kangwon National University)
Ahn, Chang-Ho (Division of forest resources, college of forest and environmental sciences, Kangwon National University)
Lee, Jae-Seon (Division of forest resources, college of forest and environmental sciences, Kangwon National University)
Choi, Yong-Eui (Division of forest resources, college of forest and environmental sciences, Kangwon National University)
Publication Information
Journal of Korean Society of Forest Science / v.96, no.1, 2007 , pp. 83-88 More about this Journal
Abstract
Micropropagation of Ulmus davidiana Planch was established via adventitious shoot formation from the segments of adventitious roots. Adventitious roots were produced directly from root segments of seedlings on a 1/2 SH medium plus various concentrations of IBA. The maximum growth of adventitious roots was observed in the presence of 2.0 mg/L IBA. After the segments of adventitious roots were cultured on various cytokinins (zeatin, 2-iP, BA, kinetin) and cytokinins plus auxin (IBA), formation of adventitious shoot was investigated. Among cytokinin treated, kinetin was the most effective on both adventitious shoot induction and number of shoots. Especially, 2.0 mg/L kinetin was the best to increase adventitious shoot induction (95.8%) and a number of shoots (8.4). Adventitious shoots were rooted on 1/2 WPM medium and the plantlets were acclimated 100% on composed soil (peatmoss : vermiculite 1 : 1).
Keywords
Ulmus davidiana Planch; adventitious shoot; cytokinin;
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