한국산 잉어과어류 칼납자루(Acheilognathus koreensis) 정자의 미세구조

Ultrastructure of Spermatozoa of a Korean Bitterling, Acheilognathus koreensis (Pisces, Cyprinidae)

  • 김구환 (대구보건대학 방사선과) ;
  • 김정기 (대구보건대학 작업치료과) ;
  • 황기주 (한국기초과학지원연구원 대구센터)
  • Kim, Kgu-Hwan (Department of Radiologic Technology, Daegu Health Colleage) ;
  • Kim, Jeong-Ki (Department of Occupational therapy, Daegu Health Colleage) ;
  • Hwang, Ki-Ju (Daegu Center, Korea Basic Science Institute)
  • 투고 : 2007.08.29
  • 심사 : 2007.11.05
  • 발행 : 2007.12.31

초록

칼납자루 Acheilognathus koreensis 정자의 미세구조를 전자현미경으로 관찰하였다. 칼납자루 정자의 미세구조는 잉어과 어류정자의 일반적인 구조와 같이 둥근 두부, 얕은 핵와, 세포의 측면에 위치한 편모구조 그리고 비 대칭적인 편모구조의 특징을 취하고 있었다. 그러나 중편에 위치한 미토콘드리아는 대부분의 잉어류에서와는 달리 융합되어 하나를 형성하고 있으며 그 주위를 막성 구조물들이 둘러싸고 있었다. 하나로 융합된 미토콘드리아를 가지는 정자의 구조는 여러개로 나타나는 것에 비하여 경골어류에서는 파생형질로 알려져 있다. 잉어류 정자에서 미토콘드리아가 융합되어 하나로 나타나는 구조는 Rodeus와 Puntungia에서 보고되어 있으며 그 주위를 막성구조물이 둘러싸고 있는 것은 납자루류에서만 보고되고 있다.

The bitterling, Acheilognathus koreensis spermatozoon has been examined by electron microscopy. The epididymal spermatozoa of A. koreensis are representing typical characteristic of cyprinid spermatozoa including the lateral insertion of flagellum, the organization of centriolar complex in shallow nuclear fossa and the asymmetrical arrangement of mitochondria. The sperm mid-piece contains a large mitochondrion characteristic enclosed by membranous vesicles. The mitochondria aspect is different from that of other cyprinid spermatozoa, which their mitochondria have a conventional aspect and never fuse to form a mitochondrial derivative. In term of sperm evolution, the fused mitochondria are regarded as the apomorphic character in comparison with the separate mitochondria. The single mitochondrion is not found in cyprinid spermatozoon except for Rodeus and Pungtungia.

키워드

참고문헌

  1. 김구환. 2006. 참갈겨니 (Teleostei, Cypriniformes, Cyprinidae)정자변형과 정자의 미세구조. 한어지, 18 : 347-354
  2. 김익수. 1997. 한국동식물도감 제37권 동물편(담수어류), 교육부, pp. 155-353
  3. 김익수.박종영. 1996. 한국산 줄종개 Cobitis striata (미꾸리과)의 정소 및 정자의 구조. 한어지, 8 : 1-8
  4. 박종영.김익수. 1996. 한국산 미꾸리과 Cobitidae (잉어목) 어류의 정자 미세구조. 한어지, 8 : 74-83
  5. Afzelius, B.A. 1972. Sperm morphology and fertilization biology: in Beatty and Gluecksohn-Wawlsch the genetics of the spermatozoon. Edinburgh and New York. NY, pp. 131-143
  6. Baccetti, B., A.G. Burrini, G. Callaini, G. Gibertini, M. Mazzini and S. Zerunian. 1984. Fish germinal cells I. Comparative spermatology of seven cyprinid species. Gamete Res., 373-396
  7. Emel'yanova, N.G. and A.P. Makeyeva. 1991a. Morphology of the gametes of the channel catfish, Ictalurus punctaus. Vopr. Ichtiol., 31 : 143-148
  8. Emel'yanova, N.G. and A.P. Makeyeva. 1991b. Ultrastructure of spermazoids of some representative catfishes. Vopr. Ichtiol., 31 : 1014-1019
  9. Guan, T.L. and B.A. Afzelius. 1991. The spermatozoon of the Chinese bitterling, Rhodeus sericeus sinensis (Cyprinidae, Teleostei). J. Submicrosc. Cytol. Pathol., 23(3): 351-356
  10. Guan, T.L. 1990. Resional specificity within plasma membrane and nuclear membrane of goldfish sperm. Acta. Biol. Sinica., 23 : 17-27
  11. Gwo, J.C., H.H. Gwo, H.C. Wu and P.W. Lin. 1996. The ultrastructure of Formosan landlockes salmon, Oncorhynchus masou formosanus, spermatozoon (Teleostei, Salmoniformcs, Salmonidac). J. Submicrosc. Cytol. Pathol., 28 : 33-40
  12. Gwo, J.C. and H.H. Gwo. 1993. Spermatogenesis in the black porgy, Acanthopagrus schegeli (Teleostei: Perciformes: Sparidae). Molecuar. Reprod. Develop., 36 :75-83 https://doi.org/10.1002/mrd.1080360111
  13. Jamieson, B.G.M. 1991. Fish evolution and systematics: Evidence from spermatozoa. New York: Cambridge University Press. pp. 135-148
  14. Kim, K.H., A.S. Kwon and Y.H. Lee. 1998. Spermatozoal ultrastructure and phylogenetic relationships of the subfamily Gobioninae (Cyprinidae, Teleostei) 2. Ultrastructure of the spermatozoa of the Korean gudgeon Squalidus chankaensis tsuchigae. Korean J. Limnol., 31(3) : 159-164
  15. Kim, K.H. and Y.H. Lee. 2000. The Ultrastructure of spermatozoa of the Ussurlan Bullhead, Leiocassis ussuriensis (Teleostei, Silurlformes, Bagridae) with Phylogenetic Considerations. Korean J. Limnol., 33(4) : 405-412
  16. Kim, Y.J., C.Y. Choi and I.S. Park. 2006. Ultrastructure of dark chub Zacco temminckii (Cyprinidae) spermatozoa. J. Fish. Sci. Technol., 9(1) : 1-6 https://doi.org/10.5657/fas.2006.9.1.001
  17. Kwon, A.S., K.H. Kim and Y.H. Lee. 1998. Ultrastructure of spermatozoa in the catfish, Silurus asotus (Teleostei, Siluriformes, Siluridae). Dev. Reprod., 2 : 75-80
  18. Lee, Y.H. 1998. Ultrastructure of spermatozoa in the bagrid catfish, Pseudobagrus fulvidraco (Teleostei, Siluriformes, Bagridae). Korean J. Electron. Microscopy., 28 :39-48
  19. Lee, Y.H. and K.H. Kim. 1998a. Spermatozoal ultrastructure and phylogenetic relationships of the subfamily Gobioninae (Cyprinidae, Teleostei) 1. Ultrastructure of the spermatozoa of the Korean Slender Gudgeon Squalidus gracilis majimae. Korean J. Electron. Microscopy., 28(1) : 63-71
  20. Lee, Y.H. and K.H. Kim. 1998b. Ultrastructure of spermatozoa in Pungtungia herzi. Dev. Reprod., 2 : 141-148
  21. Lee, Y.H. and K.H. Kim. 1999. Ultrastructure of the south torrent catfish, Liobagrus mediadiposalis (Teleostei, Siluriformes, Amblycipitidae) spermatozoon. Korean J. Limnol., 32 : 271-280
  22. Lee, Y.H. and K.H. Kim. 2001. The ultrastructure of spermatozoa of the slender catfish Silurus microdorsalis (Teleostei, Siluriformes, Siluridae) with phylogenetic considerations. J. Submicrosc. Cytol. Pathol., 33 : 329-336
  23. Mattei, X. 1991. Spermatozoon ultrastructure and its systematic implications in fishes. Can. J. Zool., 69 : 3038-3055 https://doi.org/10.1139/z91-428
  24. Mattei, X. 1988. The flagellar apparatus of spermatozoa in fish. ultrastructure and evolution. Biol. Cell., 63 : 151-158 https://doi.org/10.1016/0248-4900(88)90054-8
  25. Morisawa, S. 1979. The fine structure of the spermatozoon of the carp. Cyprinus. carpio. Bull. St. Marianne. Univ., 8 : 23-28
  26. Ohta, T. and T. Iwamatsu. 1983. Electron microscopic observations on sperm entry into eggs of the rose bitterling Rhodeus ocellatus. J. Exp. Zool., 227 : 109-119 https://doi.org/10.1002/jez.1402270115
  27. Quagio-Gassiotto, I., M.C. Gameiro, T. Schneider, L.R. Malabarba and C. Oliveira. 2003. Spermiogenesis and spermatozoa ultrastructure in five species of the Curimatidae with some considerations on spermatozoal ultrastructure in the Characiformes. Neotropical. Ichthy.,1 : 35-45 https://doi.org/10.1590/S1679-62252003000100004