Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Development and Characterization of a Specific Anti-Caveolin-1 Antibody for Caveolin-1 Functional Study in Human, Goat and Mouse

  • Ke, Meng-Wei (Department of Animal Science and Technology, National Taiwan University) ;
  • Jiang, Yan-Nian (Department of Animal Science and Technology, National Taiwan University) ;
  • Li, Yi-Hung (Department of Animal Science and Technology, National Taiwan University) ;
  • Tseng, Ting-Yu (Department of Animal Science and Technology, National Taiwan University) ;
  • Kung, Ming-Shung (Department of Animal Science and Technology, National Taiwan University) ;
  • Huang, Chiun-Sheng (Department of Surgery, National Taiwan University Hospital and National Taiwan University of College of Medicine) ;
  • Cheng, Winston Teng-Kuei (Department of Animal Science and Technology, National Taiwan University) ;
  • Hsu, Jih-Tay (Department of Animal Science and Technology, National Taiwan University) ;
  • Ju, Yu-Ten (Department of Animal Science and Technology, National Taiwan University)
  • Received : 2006.11.17
  • Accepted : 2007.02.27
  • Published : 2007.06.01
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Abstract

Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-1 protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-1 and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells.

Keywords

References

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