Molecules and Cells

  • 제24권2호
  • /
  • Pages.240-246
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  • 2007
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Identification of Critical Residues for Plasminogen Binding by the αX I-domain of the β2 integrin, αXβ2

  • Gang, Jongyun (Divisions of Science Education and Biology, Research Institute of Life Sciences, Kangwon National University) ;
  • Choi, Jeongsuk (Divisions of Science Education and Biology, Research Institute of Life Sciences, Kangwon National University) ;
  • Lee, Joo Hee (Divisions of Science Education and Biology, Research Institute of Life Sciences, Kangwon National University) ;
  • Nham, Sang-Uk (Divisions of Science Education and Biology, Research Institute of Life Sciences, Kangwon National University)
  • 투고 : 2007.04.05
  • 심사 : 2007.05.23
  • 발행 : 2007.10.31
⟨ 이전 논문 다음 논문 ⟩

초록

The ${\beta}2$ integrins on leukocytes play important roles in cell adhesion, migration and phagocytosis. One of the ${\beta}2$ integrins, ${\alpha}X{\beta}2$ (CD11c/CD18), is known to bind ligands such as fibrinogen, Thy-1 and iC3b, but its function is not well characterized. To understand its biological roles, we attempted to identify novel ligands. The functional moiety of ${\alpha}X{\beta}2$, the ${\alpha}X$ I-domain, was found to bind plasminogen, the zymogen of plasmin, with moderate affinity ($1.92{\times}10^{-6}M$) in the presence of $Mg^{2+}$ or $Mn^{2+}$. The ${\beta}D-{\alpha}5$ loop of the ${\alpha}X$ I-domain proved to be responsible for binding, and lysine residues ($Lys^{242}$, $Lys^{243}$) in the loop were the most important for recognizing plasminogen. An excess amount of the lysine analog, 6-aminohexanoic acid, inhibited ${\alpha}X$ I-domain binding to plasminogen, indicating that binding is lysine-dependent. The results of this study indicate that leukocytes regulate plasminogen activation, and consequently plasmin activities, through an interaction with ${\alpha}X{\beta}2$ integrin.

키워드

과제정보

연구 과제 주관 기관 : Kangwon National University

참고문헌

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