Molecules and Cells

  • 제23권1호
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  • Pages.94-99
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  • 2007
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

PMA Activates Stat3 in the Jak/Stat Pathway and Induces SOCS5 in Rat Brain Astrocytes

  • Hwang, Mi-Na (Research Institute, National Cancer Center) ;
  • Kim, Kwang Soo (Department of Pharmacology, Ajou University School of Medicine) ;
  • Choi, Yo-Woo (Research Institute, National Cancer Center) ;
  • Jou, Ilo (Department of Pharmacology, Ajou University School of Medicine) ;
  • Yoon, Sungpil (Research Institute, National Cancer Center)
  • 투고 : 2006.08.24
  • 심사 : 2006.12.07
  • 발행 : 2007.02.28
⟨ 이전 논문 다음 논문 ⟩

초록

Suppressors of cytokine signaling (SOCS) family members are negative feedback regulators of the Jak/Stat pathway, which is an essential inflammatory signaling pathway. We investigated expression of eight members of the SOCS family in rat astrocytes, using two inflammatory stimulants, PMA and IFN-${\gamma}$. Only a few SOCS genes were induced by both stimulants, and we detected an increase in SOCS5 protein with PMA. PMA activated the Jnk, Erk, p38, and Jak/Stat signal pathways. In addition, it increased the level of activated-Stat3 resulting from tyrosine phosphorylation. A gel-shift assay showed that a protein in nuclear extracts from PMA-treated cells was able to bind to Stat binding elements. These results suggest that activated Stat3 binds to SOCS promoters and leads to their transcriptional induction.

키워드

과제정보

연구 과제 주관 기관 : National Cancer Center

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