Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

  • Cho, Eun-Seong (Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University) ;
  • Lee, So-Yeong (Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University) ;
  • Park, Jae-Yong (Department of Physiology, Institute of Health Science and Medical Research Center for Neural Dysfunction, Gyeongsang National University College of Medicine) ;
  • Hong, Seong-Geun (Department of Physiology, Institute of Health Science and Medical Research Center for Neural Dysfunction, Gyeongsang National University College of Medicine) ;
  • Ryu, Pan-Dong (Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University)
  • Published : 20070300
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Abstract

Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.

Keywords

Acknowledgement

The authors thank Dr. Seung Yub Shin, Mr. Tae Hee Han, and Mr. Jian Hua Yang for their technical assistance. This work was supported by a grant (R01-2002-000-00128-0) from the Basic Research Program of the Korea Science and Engineering Foundation, and was performed as a portion of the Master's degree of the late Dr. Eun Seong Cho.

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