Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Extracellular Secretion of a Maltogenic Amylase from Lactobacillus gasseri ATCC33323 in Lactococcus lactis MG1363 and its Application on the Production of Branched Maltooligosaccharides

  • Cho, Mee-Hyun (Department of Food Science & Biotechnology and Institute of Life Science & Resources, KyungHee University) ;
  • Park, Sang-Eun (Department of Food Science & Biotechnology and Institute of Life Science & Resources, KyungHee University) ;
  • Lee, Myung-Hun (Department of Food Science & Biotechnology and Institute of Life Science & Resources, KyungHee University) ;
  • Ha, Suk-Jin (Department of Food Science & Biotechnology and Institute of Life Science & Resources, KyungHee University) ;
  • Kim, Hae-Yeong (Department of Food Science & Biotechnology and Institute of Life Science & Resources, KyungHee University) ;
  • Kim, Myo-Jeong (Department of Food Science and Biotechnology, University of Inje) ;
  • Lee, Sung-Joon (Division of Food Science, College of Life Science & Biotechnology, Korea University, and Institute of Biomedical Science & Food Safety) ;
  • Madsen, Soren M. (Bioneer A/S) ;
  • Park, Cheon-Seok (Department of Food Science & Biotechnology and Institute of Life Science & Resources, KyungHee University)
  • Published : 2007.09.30
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Abstract

A maltogenic amylase gene from Lactobacillus gasseri ATCC33323 (LGMA) was expressed in Lactococcus lactis MG1363 using the P170 expression system. The successful production of recombinant LGMA (rLGMA) was confirmed by the catalytic activity of the enzyme in liquid and solid media. The N-terminal amino acid sequencing analysis of the rLGMA showed that it was Met-Gln-Leu-Ala-Ala-Leu-, which was the same as that of genuine protein, meaning the signal peptide was efficiently cleaved during secretion to the extracellular milieu. The optimal reaction temperature and pH of rLGMA ($55^{\circ}C$ and pH 5, respectively) and enzymatic hydrolysis patterns on various substrates (${\beta}$-cyclodextrin, starch, and pullulan) supported that rLGMA was not only efficiently secreted from the Lactococcus lactis MG1363 but was also functionally active. Finally, the branched maltooligosaccharides were effectively produced from liquefied com starch, by using rLGMA secreted from Lactococcus lactis, with a yield of 53.1%.

Keywords

References

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