Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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PCR-based Specific Detection of Ralstonia solanacearum by Amplification of Cytochrome c1 Signal Peptide Sequences

  • Kang, Man-Jung (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Lee, Mi-Hee (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Shim, Jae-Kyung (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Seo, Sang-Tae (Korea Forest Research Institute) ;
  • Shrestha, Rosemary (Central Department of Botany, Tribhuvan University) ;
  • Cho, Min-Seok (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Hahn, Jang-Ho (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Park, Dong-Suk (National Institute of Agricultural Biotechnology, Rural Development Administration)
  • Published : 2007.11.30
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Abstract

A polymerase chain reaction (PCR)-based method was developed to detect the DNA of Ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. One pair of primers (RALSF and RALSR), designed using cytochrome c1 signal peptide sequences specific to R. solanacearum, produced a PCR product of 932 bp from 13 isolates of R. solanacearum from several countries. The primer specificity was then tested using DNA from 21 isolates of Ralstonia, Pseudomonas, Burkholderia, Xanthomonas, and Fusarium oxysporum f. sp. dianthi. The specificity of the cytochrome c1 signal peptide sequences in R. solanacearum was further confirmed by a DNA-dot blot analysis. Moreover, the primer pair was able to detect the pathogen in artificially inoculated soil and tomato plants. Therefore, the present results indicate that the primer pair can be effectively used for the detection of R. solanacearum in soil and host plants.

Keywords

References

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