Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Nuclear SSU and Plastid rbcL Genes and Ultrastructure of Mallomonas caudata (Synurophyceae) from Korea

한국산 Mallomonas caudata (Synurophyceae)의 미세구조, 핵 SSU 그리고 색소체 rbcL 유전자

  • Published : 2007.09.30
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Abstract

Despite geographic barriers such as oceans, many freshwater algal species inhabit different continents of the world. A unicellular freshwater alga, Mallomonas caudeata, commonly occurring in Asia, Europe, and America of the northern Hemisphere, is closely related to human life such as monitoring blooms and defecting changes in climates. In order to demonstrate its occurrence in Korea and to infer its phylogeny, we sequenced nuclear SSU and plastid rbcL genes from isolates collected in six different reservoirs. We have also investigated transmission electron microscopy of the Korean isolates. SSU sequences of the species from Korea and USA were almost identical, having pair-wise divergences of 0.06% in SSU and 0.45% in rbcL. Both gene trees revealed that the species was clearly separated from other species of the genus, while the genus was not monophyletic. Rhizoplasts are composed of microfibrils organised in striated rootlets attached to the multilayered plate of basal bodies and arranged on the surface of the nucleus at their distal ends. The rhizoplast constitutes a basal body-nucleus connector similar to that of typical Synurophyceas. The results that Mallomonas was not supported by both SSU and rbeL data sets require a further study with additional taxon sampling.

해양 같은 지리적 장벽에도 불구하고, 많은 담수조류는 세계의 다른 대륙에 서식하고 있다. 단세포 담수조류, Mallomonas caudata는 북반구의 미국, 유럽, 및 아시아에서 흔하며, 기후변화 감지나 수화현상 감시 같은 인간생활에 밀접하게 관련되어 있다. 본 종의 계통과 국내출현을 설명하기 위해 여섯 군데의 저수지에서 채집된 균주로부터 엽록체 rbcL과 핵 SSU유전자를 염기서열 분석하였다. 또한, 한국산 균주의 전자현미경적 구조를 조사하였다. 한국과 미국산 종의 SSU염기서열은 0.06%가, rbcL은 0.45%의 차이로 거의 동일하였다. 두 유전자를 이용한 계통수에서 본 종은 속의 다른 종들과 분명히 분리되지만, 단계통군은 아니었다. 근형질은 기저체의 다층판에 부착된 가로무늬의 microfibril들로 구성되어 있었으며, microfibril들의 끝은 핵의 표면 위에 배열되어 있었다. 이 근형질은 Synurophyceae에서 전형적으로 보이는 기저체-핵 연결자이다. Mallomonas가 SSU와 rbcL자료에 의해 지지되지 않는 결과는 분류군 추가와 함께 좀더 연구되어야 할 것으로 사료된다.

Keywords

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