Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Serological Analysis of the Antigenicity during Cultivation of Streptomyces Strains

Streptomyces 속 균주들의 생장에 따른 외피 항원성 변화의 혈청학적 분석

  • Kim, Jae-Heon (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Jo, Sung-Kee (Radiation Research Center for Biotechnology, Korea Atomic Energy Research Institute)
  • 김재헌 (단국대학교 미생물학 전공 및 기초과학연구소) ;
  • 조성기 (한국원자력연구원 방사선생명공학연구센터)
  • Published : 2007.12.30
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Abstract

The changes in antigenicity during cultivation of streptomycetes were determined by immunodiffusion assay and indirect ELISA. New precipitin lines in immunodiffusion assay began to appear in the growth period of the soluble pigment production and became thickened thereafter. The increase in the antigenicity was also confirmed by ELISA. The antigenic development was relatively weak for S. lavendulae and S. viridochromogenes while that was strong for S. lavendulae and S. viridochromogenes. The results indicated that Streptomyces strains, even though not proved for some strains, changed the compositions of cell envelope during submerged growth and this could be estimated quantitatively by serological method.

Streptomyces 균주들의 진탕배양 시간에 따른 항원성 변화를 면역확산 침강법과 간접 ELISA법으로 측정하였다. 각 균주들에서 모두 색소 생산 시기에 나타난 새로운 침강선이 배양시간이 경과되면서 진해지는 양상을 보였다. S. coelicolor와 S. griseus의 항원성 변화는 상대적으로 약하게 나타난 반면 S. lavendulae와 S. viridochromogenes의 항원성 변화는 강하게 나타났다. 이 결과는 균주에 따라 정도의 차이는 있지만 Streptomyces 균체의 진탕배양에서의 생장이 균체의 외피 성분의 변화를 수반하며 이를 정량적으로 분식할 수 있음을 나타낸다.

Keywords

References

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