Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification and Molecular Characterization of Novel cry1-Type Toxin Genes from Bacillus thuringiensis K1 Isolated in Korea

  • Li Ming Shun (State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University) ;
  • Choi Jae-Young (Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Roh Jong-Yul (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Shim Hee-Jin (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kang Joong-Nam (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kim Yang-Su (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Wang Yong (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Yu Zi Niu (State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University) ;
  • Jin Byung-Rae (Division of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Je Yeon-Ho (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
  • Published : 2007.01.31
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Abstract

To clone novel cry1-type genes from the Bacillus thuringiensis K1 isolate, about 2.4-kb-long PCR fragments were amplified with two primer sets of ATG1-F/N400-R and 1BeATG1-F/N400-R. Using PCR-RFLP, three novel cry1-type genes, cry1-1, cry1-7, and cry1-44, were obtained from B. thuringiensis K1 and the complete coding sequences of these novel genes were analyzed. The Cry1-1, Cry1-7, and Cry1-44 proteins showed maximum similarities of about 78.0%, 99.7%, and 91.0% with the Cry1Ha1, Cry1Be1, and Cry1Ac2 proteins, respectively. These novel cry1-type genes were expressed using a baculovirus expression vector system and their insecticidal activities were investigated. Whereas all three novel genes were toxic to Plutella xylostella larvae, only Cry1-1 showed insecticidal activity against Spodoptera exigua larvae.

Keywords

References

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