Journal of Microbiology

  • 제45권4호
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  • Pages.339-343
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  • 2007
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Screening of Genes Related to Methylglyoxal Susceptibility

  • Kim, In-Sook (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Kim, Joon-Ho (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Min, Bum-Chan (Analytical Science Center, Samyang Central R&D Institute) ;
  • Lee, Chang-Han (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Park, Chan-Kyu (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • 발행 : 2007.08.30
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초록

Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression.

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참고문헌

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