농약과학회지 (The Korean Journal of Pesticide Science)

  • 제11권2호
  • /
  • Pages.117-124
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  • 2007
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지

포식성 무당벌레(Harmonia axyridis) 난(卵)의 일부살충제와 살균제에 대한 esterase 활성 및 산란율, 부화율 조사

Evaluation of Toxicity of 23 Pesticides against Harmonia axyridis (Coleoptera: Coccinellidae) Eggs and Adults: Effect on Esterase Activity, Hatchability, and Fecundity

  • 조세열 ;
  • 박영만 (강원대학교 BT 특성화 학부 식물생명공학) ;
  • 박용철 (강원대학교 BT 특성화 학부 식물생명공학)
  • Cho, Sae-Youll (Division of Metabolism, Endocrinology, and Diabetes, University of Michigan) ;
  • Park, Young-Man (Department of Plant Biotechnology Program, Division of Biotechnology, School of Biotechnology Kangwon National University) ;
  • Park, Yong-Chul (Department of Plant Biotechnology Program, Division of Biotechnology, School of Biotechnology Kangwon National University)
  • 발행 : 2007.06.30
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초록

포식성 무당벌레(Harmonia axyridis)의 난(卵)을 대상으로 저독성 약제를 선발하기 위해 농약 처리 후 esterase 활성에 대해 조사하였다. 무당벌레의 난에는 3개의 esterase bands가 존재하였고 esterase의 활성에 영향을 주는 살충제(Methidation, Chlorpyrifos, Phenthoate)는 유기인계인 것으로 나타났다. 살균제로는 트리아졸계 계통의 Hexaconazole과 Triflumizole 그리고 피리미딘계의 Nuarimol에서 esterase의 활성 저해가 관찰되었다. 선발된 농약을 바탕으로 무당벌레의 산란율과 부화율을 조사하였다. Esterase에 강한 활성저해를 보였던 농약에서는 산란율과 부화율도 낮게 나타났으며, esterase에 약하게 혹은 전혀 영향을 주지 않았던 농약에서도 정도 차이는 있으나 산란율과 부화율에 영향을 주는 것으로 관찰되었다.

Esterase activity was observed after pesticides treatment in eggs of H. axyridis to select low toxicity pesticide. Egg esterases of H. axyridis were examined using an esterase substrate(${\alpha}$-naphthyl acetate). Three esterase isozymes were detected and the activities were inhibited by organophosphorus insecticide (Chlorpyrifos and Phenthoate), organochlorine insecticide(Methidation), triazole fungicide(Hexaconazole and Triflumizole), and pyrimidine fungicide(Nuarlmol). Fecundity and hatchability in adults and eggs of H. axyridis were examined on selected pesticides. Fecundity and hatchability were significantly reduced from H. axyridis adults and eggs treated with the pesticides and the fungicides showed strong inhibition of esterase isozymes activities. However, we also observed the pesticides and the fungicides showed low or non-inhibition of esterase isozymes activities affected on fecundity and hatchability in adults and eggs.

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