대한두경부종양학회지 (Korean Journal of Head & Neck Oncology)

  • 제23권1호
  • /
  • Pages.21-25
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  • 2007
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  • 1229-5183(pISSN)
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  • 2586-2553(eISSN)
대한두경부종양학회 (The Korean Society for Head and Neck Oncology)
권호 표지

편도암의 발암 원인으로 Human Papilloma Virus를 통한 발암 기전과의 상관 관계

Correaltion of Human Papilloma Virus Infection Status with Tonsillar Squamous Cell Carcinoma

  • 김세헌 (연세대학교 의과대학 이비인후과학교실) ;
  • 변형권 (연세대학교 의과대학 이비인후과학교실) ;
  • 천제영 (연세대학교 의과대학 이비인후과학교실) ;
  • 박영민 (연세대학교 의과대학 이비인후과학교실) ;
  • 정진세 (연세대학교 의과대학 이비인후과학교실) ;
  • 이소윤 (연세대학교 의과대학 이비인후과학교실)
  • Kim, Se-Heon (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Byun, Hyung-Kwon (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Cheon, Jei-Young (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Park, Young-Min (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Jung, Jin-Sei (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Lee, So-Yoon (Department of Otorhinolaryngology, Yonsei University College of Medicine)
  • 발행 : 2007.05.31
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초록

Background：Squamous cell carcinoma(SCC)of the palatine tonsils represents approximately 15-23% of all intraoral SCC. The most frequently reported risk factors for oropharyngeal cancer are smoking and alcohol. In a recent overview of HPV and tonsillar squamous cell carcinoma(TC), 51% contained HPV DNA, and HPV-16 being the most frequent type. We aimed to clarify whether HPV directly effects on the oncogenesis and biologic behavior of TC by comparison with infection prevalence, and physical status of virus. Material and Method：We used HPV genotyping DNA chip(Biocore, Korea, Seoul) arrayed by multiple oligonucleotide probes of L1 sequence of 26 types of HPV and HPV genotypes are identified by fluorescence scanner. The copy numbers of HPV E2 and E6 open reading frames(ORF) were assessed using a TaqMan-based 5'-exonuclease quantitative real-time PCR assay. The ratio of E2 to E6 copy numbers was calculated to determine the physical status of HPV-16 viral gene. Results：We observed a significant difference in HPV prevalence between 52 TCs and 69 CFTs(73.1% vs. 11.6%), and most of the HPVs were type 16(87.2%)and non-episomal(94.1%) state. Conclusions：This study regarding HPV infection prevalence and mechanism in the largest population of palatine tonsillar squamous cell carcinoma with chronic follicular tonsillitis revealed significant difference pf HPV prevalence between TC and CFT. Most of HPV were 16 type and integrated or mixed, HPV-16 integration could be directly related to tonsillar carcinogenesis.

키워드

참고문헌

  1. Syrjanen S: HPV infections and tonsillar carcinoma. J Clin Pathol. 2004;57:449-455 https://doi.org/10.1136/jcp.2003.008656
  2. Munger K, Werness BA, Dyson N, Phelps WC, Harlow E, Howley PM: Complex formation of human papillomavirus E7 protrein with the retinoblastoma tumor suppressor gene product. EMBO J. 1989;8:4099-4105
  3. Scheffner M, Werness BA, Huibregtse JM, Levine AJ, Howley PM: The E6 oncoprotein encoded by human papillomavirus type 16 and 18 promotes the degradation of p53. Cell. 1990;63:1129 -1136 https://doi.org/10.1016/0092-8674(90)90409-8
  4. Peitsaro P, Johansson B, Syrjanen S: Integrated human papillomavirus type 16 is frequently found in cervical cancer precursors as demonstrated by a novel quantitative realtime PCR technique. J Clin Microbiol. 2002;40:886-891 https://doi.org/10.1128/JCM.40.3.886-891.2002
  5. Kalantari M, Karlsen F, Kristensen G, Holm R, Hagmar B, Johansson B: Disruption of the E1 and E2 reading frames of HPV 16 in cervical carcinoma is associated with poor prognosis. Int J Gynecol Pathol. 1998;17:146-153 https://doi.org/10.1097/00004347-199804000-00009
  6. Vernon SD, Unger ER, Miller DL, Lee DR, Reeves WC: Association of human papillomavirus type 16 integration in the E2 gene with poor disease-free survival from cervical cancer. Int J Cancer. 1997;74:50-56 https://doi.org/10.1002/(SICI)1097-0215(19970220)74:1<50::AID-IJC9>3.0.CO;2-#
  7. Mellin H, Dahlgren L, Munik-Wikland E, et al: Human papillomavirus type 16 is episomal and a high viral load may be correlated to better prognosis in tonsillar cancer. Int J Cancer. 2002;102:152-158 https://doi.org/10.1002/ijc.10669
  8. Koskinen WJ, Chen RW, Leivo I, et al: Prevalence and physical status of human papillomavirus in squamous cell carcinomas of the heand and neck. Int J Cancer. 2003;107:401-406 https://doi.org/10.1002/ijc.11381
  9. Venuti A, Manni V, Morello R, De Marco F, Marzetti F, Marcante ML: Viral integration into the host genome occurred in 43% of cases of HPV-16 and in 20% of cases of HPV-6. Viral RNA expression was detected by reverse transcription-PCR only in HPV-16 positive tumors. J Med Virol. 2000;60:396-402 https://doi.org/10.1002/(SICI)1096-9071(200004)60:4<396::AID-JMV6>3.0.CO;2-V
  10. Si HX, Tsao SW, Poon CSP, Wong YC, Cheung ALM: Physical status of HPV-16 in esophageal squmaous cell carcinoma. J Clin Virol. 2005;32:19-23 https://doi.org/10.1016/j.jcv.2004.04.004
  11. Kalantari M, Karlsen F, Kristensen G, Holm R, Hagmar B, Johansson B: Disruption of the E1 and E2 open reading frames of HPV 16 in cervical carcinoma is associated with poor prognosis. Int J Gynecol Pathol. 1998;17:146-153 https://doi.org/10.1097/00004347-199804000-00009
  12. Peitsaro P, Johansson B, Syrjanen S: Integrated human papillomavirus type 16 is frequently found in cervical cacner precursors as demonstrated by a novel quantitative real-time PCR technique. J Clin Microbiol. 2002;40:886-891 https://doi.org/10.1128/JCM.40.3.886-891.2002