Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidant Properties of Water Extract from Acorn

  • Yin, Yu (School of Biotechnology, Kangwon National University) ;
  • Heo, Seong-Il (School of Biotechnology, Kangwon National University) ;
  • Jung, Mee-Jung (School of Biotechnology, Kangwon National University) ;
  • Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
  • Published : 2007.06.30
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Abstract

Antioxidant and anti-diabetic activities of acorn were evaluated by its potential for scavenging stable DPPH free radical, inhibition of lipid peroxidation, reducing power, and inhibiton of ${\alpha}-glucosidase\;and\;{\alpha}-amylase$. The water extract of acorn exhibited strong antioxidant and antidiabetic related activities in the tested model systems. Solvent fractionation of the water extract revealed that the water fraction and the EtOAc fraction had strong antioxidant activity, and inhibitory activity on ${\alpha}-glucosidase\;and\;{\alpha}-amylase$. The water fraction exhibited higher DPPH radical scavenging activity ($EC_{50}=7.19{\mu}g/mL$) than that of ${\alpha}-tocopherol\;(EC_{50}=7.59{\mu}g/mL)$. It is considered that water extract of acorn has the potential for natural antioxidant and anti-diabetic products.

Keywords

References

  1. Athukorala Y, Kim KN, and Jeon YJ (2006) Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava Food Chem Toxicol 44, 1065-1074 https://doi.org/10.1016/j.fct.2006.01.011
  2. Chiou JW (1989) The antioxidant activity and the chemical structure of selected components acorns and their potential use as inhibitors of milk oxidation. A Dissertation of Cornell University, USA
  3. Inzucchi SE (2002) Oral antihyperglycemic therapy for type 2 diabetes: scientific review. JAMA 287, 360-372 https://doi.org/10.1001/jama.287.3.360
  4. Kilani S, Ammar RB, Bouhlel I, Abdelwahed A, Hayder N, Mahmoud A, Ghedira K, and Chekir-Ghedira L (2005) Investigation of extracts from (Tunisian) Cyperus rotundus as antimutagens and radical scavengers. Environ Toxicol Phar 20, 478-484 https://doi.org/10.1016/j.etap.2005.05.012
  5. Kim YM and Jeong YK (2005) Inhibitory effect of pine extract on $\alpha$-glucosidase activity and postprandial hyperglycemia. Nutrition 21, 756-761 https://doi.org/10.1016/j.nut.2004.10.014
  6. Lee MH, Jeang JH, and Oh MJ (1992) Antioxidant activity of galic acid in acorn extract. J Korean Soc Food Nutr 21, 639-700
  7. Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193, 265-275
  8. Maeda K, Kakabayashi S, and Matsubara H (1985) Complete amino acid sequence of an $\alpha$-amylase inhibitor in wheat kernel (0.10-inhibitor). Biochem Biophys Acta 828, 213-221 https://doi.org/10.1016/0167-4838(85)90299-7
  9. Masao H, Yang XW, Miyashiro H, and Nabma T (1993) Inhibitory effects of monomeric and dimeric phenylpropanoids from mice on lipid peroxidation in vivo and in vitro. Phytother Res 7, 395-401 https://doi.org/10.1002/ptr.2650070603
  10. Mihara M, Uchiyama M, and Fukuzawa K (1980) Thiobarbituric acid value on fresh homogenate of rats as a parameter of lipid peroxidation in aging, CCl4 intoxication, and vitamin E deficiency. Biochem Med 23, 302-311 https://doi.org/10.1016/0006-2944(80)90040-X
  11. Oki T, Masuda M, Furuta S, Nishibia Y, Terahara N, and Suda I (2002) Involvement of anthrocyanins and other phenolic compounds in radical scavenging activity of purple-fleshed sweet potato cultivas. Food Chem 67, 1752-1756
  12. Oyaizu M (1986) Studies on products of browning reaction prepared from glucosamine. Japanese J Nutr 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  13. Pelagic V (1893) Folks teacher IV (in Serbian), Matica Srpska, Novi Sad
  14. Rakic S, Povrenovic D, Tes¡evic V, Simic M, and Maletic R (2006) Oak acorn, polyphenols and antioxidant activity in functional food. J Food Eng 74, 416-423 https://doi.org/10.1016/j.jfoodeng.2005.03.057
  15. Siriwardhana SSKW and Shahidi F (2002) Antiradical activity of extracts of almond and its by-products. J Am Oil Chem Soc 79, 903-908 https://doi.org/10.1007/s11746-002-0577-4
  16. Toeller M (1994) Alpha-glucosidase inhibitors in diabetes: efficacy in NIDDM. subjects. Eur J Clin Invest 24, 31-35 https://doi.org/10.1111/j.1365-2362.1994.tb02253.x
  17. Wong SH, Knight JA, Hopfer SM, Zaharia O, Leach CN, and Sunderman FW (1987) Lipoperoxide in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct. Clin Chem 33, 214-220
  18. Yki-Jarvinen H (1997) Acute and chronic effects of hyperglycemia on glucose metabolism. Diabet Med 33, 579-585
  19. Yoshiyuki K, Michinori K, Tadato T, Shigeru A, and Hiromichi O (1981) Studies on scutelariae radix, IV. Effects on lipid peroxidation in rat liver. Chem Pharml Bull 29, 2610-2617 https://doi.org/10.1248/cpb.29.2610