Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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The Anticancer Properties of Lunasin Peptide from Aged Callus Induced by the Soybean Tissue Culture

  • Park, Jae-Ho (School of Bioresources, Andong National University) ;
  • Jeong, Jin-Boo (School of Bioresources, Andong National University) ;
  • De Lumen, Ben O. (Division of Nutritional Science & Toxicology, University of California) ;
  • Jeong, Hyung-Jin (School of Bioresources, Andong National University)
  • Published : 2007.12.30
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Abstract

Lunasin is small subunit peptide of coded from Gm2S-1 gene in soybean. It has been previously demonstrated that lunasin is a novel and promising cancer preventive peptide. Lunasin peptide is found only in the seed and not other tissues. And lunasin peptide starts to appear at 5 weeks after flowering and remains in the mature seed. We report here firstly lunasin peptide identified from soybean callus induced by the tissue culture and demonstrate its anticancer properties. The lunasin was identified and purified from soybean callus aged for 6 months. The callus lunasin($1{\mu}M$) inhibited the acetylation of histone H3 and H4 by 58.8% and 56.5%, respectively. And it fully inhibited foci formation compared to the values of the positive control(no lunasin) and negative control(no MCA). Purified lunasin was able to internalize into the cell and localized in the nucleus.

Keywords

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