Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Hepatoprotective compounds of Cassiae Semen on tacrine-induced cytotoxicity in Hep G2 cells

결명자의 타크린으로 유발한 간 세포독성 보호 성분

  • Byun, Erisa (College of Pharmacy, Wonkwang University) ;
  • Jeong, Gil-Saeng (Institute for Radiological Imaging Science, Wonkwang University) ;
  • An, Ren-Bo (College of Pharmacy, Yanbian University) ;
  • Li, Bin (College of Pharmacy, Wonkwang University) ;
  • Lee, Dong-Sung (College of Pharmacy, Wonkwang University) ;
  • Ko, Eun-Kyung (College of Pharmacy, Wonkwang University) ;
  • Yoon, Kwon-Ha (Institute for Radiological Imaging Science, Wonkwang University) ;
  • Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
  • 변에리사 (원광대학교 약학대학) ;
  • 정길생 (원광대학교 익산방사선연구센터) ;
  • 안인파 (연변대학교 약학대학) ;
  • 리빈 (원광대학교 약학대학) ;
  • 이동성 (원광대학교 약학대학) ;
  • 고은경 (원광대학교 약학대학) ;
  • 윤권하 (원광대학교 익산방사선연구센터) ;
  • 김윤철 (원광대학교 약학대학)
  • Published : 2007.12.31
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Abstract

Tacrine is an acetylcholinesterase inhibitor that is approved for the treatment of Alzheimer's disease. However, tacrine treatment for Alzheimer's disease results in reversible hepatotoxicity in 30-50% of patients, which seriously limits its clinical use. Accordingly, the identification of constituents in natural products that have protective effects on tacrine-induced hepatotoxicity would be valuable. In the present study, an immortalized human hepatoma cell line, HepG2 was employed to screen for agents that protect against tacrine-induced hepatotoxicity. The bioassay-guided fractionation of water extract of Cassiae Semen furnished two anthraquinones, aurantio-obtusin (1) and obtusifolin (2). Compounds 1 and 2 showed hepatoprotective effects with the protection ratio values of 55.3 +/- 0.5% and 41.2 +/- 0.8% at $160{\mu}M$, respectively.

Keywords

References

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