The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Analysis of the Complete Genome Sequence of Zucchini yellow mosaic virus strain A Isolated from Hollyhock

  • Published : 2007.12.31
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Abstract

The complete genome sequence of Zucchini yellow mosaic virus stain A (ZYMV-A) isolated from a hollyhock (Althaea rosea) was determined by using RT-PCR with a series of primer sets. The virus genome consisted of 9593 nucleotides (nt), excluding the poly(A) tract at 3' terminus of the virus genome, with 5' and 3' untranslated region of 139 and 211 nt, respectively. The deduced polyprotein of ZYMV-A consisted of 3080 amino acid (aa) residues and was 351 kDa in molecular weight. All proteolytic cleavage sites of the polyprotein of ZYMV-A were compared with those of ZYMV strains, which showed the cleavage sites were conserved among ZYMV strains. The HC-Pro contained the KITC and PTK motifs, and the DAG motif was located at CP ORF of ZYMV-A, suggesting that ZYMV-A is aphid-transmissible. Phylogenetic tree analysis based on the complete genome among ZYMV strains or CP ORFs with other potyviruses showed ZYMV strains formed a distinct group. These results clearly confirmed that ZYMV-A was another distinct strain in ZYMV population at molecular level.

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References

  1. Atreya, P. L., Atreya, C. D. and Pirone, T. P. 1991. Amino acid substitutions in the coat protein results in loss of insect transmissibility of a plant virus. Proc. Natl. Acad. Sci. USA 88:7887-7891
  2. Atreya, C. D. and Pirone, T. P. 1993. Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proc. Natl. Acad. Sci. USA 90:11919-12388
  3. Baker, C. A., Hiebert, E., Marlow, G C. and Wisler, G C. 1992. Comparative sequence analysis of the Reunion isolate of zucchini yellow mosaic virus. Phytopathology 82: 1176
  4. Balint, R., Plooy, I. and Steele, C. 1990. The nucleotide sequence of zucchini yellow mosaic potyvirus. Abstr. VIIIth Int Congress Virol. 8:84-107
  5. Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. J. Virol. Methods 83:67-73 https://doi.org/10.1016/S0166-0934(99)00106-8
  6. Choi, S. K., Yoon, J. Y., Ryu, K. H., Choi, J. K., Palukaitis, P. and Park, W. M. 2002a. Systemic movement of a movement-deficient strain of Cucumber mosaic virus in zucchini squash is facilitated by a cucurbitinfecting potyvirus, J. Gen. Virol. 83:3173-3178 https://doi.org/10.1099/0022-1317-83-12-3173
  7. Choi, S. K., Yoon, J. Y., Ryu, K. H., Choi, J. K., Palukaitis, P. and Park, W. M. 2002b. First report of Zucchini yellow mosaic virus on hollyhock (Althaea rosea). Plant Pathol. J. 18: 121-125 https://doi.org/10.5423/PPJ.2002.18.3.121
  8. Desbiez, C, Gal-On, A., Girard, M., Wipf-Scheibel, C. and Lecoq, H. 2003. Increase in Zucchini yellow mosaic virus symptom severity in tolerant zucchini cultivars is related to a point mutation in P3 protein and is associated with a loss of relative fitness on susceptible plants. Phytopathology 93: 1478-1484 https://doi.org/10.1094/PHYTO.2003.93.12.1478
  9. Desbiez, C., Wipf-Scheibel, C. and Lecoq, H. 2002. Biological and serological variability, evolution and molecular epidemiology of Zucchini yellow mosaic virus (ZYMV, Potyvirus) with special reference to Caribbean islands. Virus Res. 85:5-16 https://doi.org/10.1016/S0168-1702(02)00013-8
  10. Gal-On, A. 2000. A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptom expression in cucurbits and exhibits protection against the severe homologous virus. Phytopathology 90:467-473 https://doi.org/10.1094/PHYTO.2000.90.5.467
  11. Gal-On, A., Antignus, Y., Rosner, A. and Raccah, B. 1992. A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication. J. Gen. Virol. 73:2183-2187 https://doi.org/10.1099/0022-1317-73-9-2183
  12. Glasa, M., Svoboda, J. and Novakova, S. 2007. Analysis of the molecular and biological variability of Zucchini yellow mosaic virus isolates from Slovakia and Czech Republic. Virus Genes 35:415-421 https://doi.org/10.1007/s11262-007-0101-4
  13. Hull, R. 2002. Matthews' Plant Virology, 4th. ed. San Diego; Academic Press, USA
  14. Kwon, S.-W., Kim, M.-S., Choi, H.-S. and Kim, K-H. 2005. Biological characteristics and nucleotide sequences of three Korean isolates of Zucchini yellow mosaic virus. J. Gen. Plant Pathol. 71:80-85 https://doi.org/10.1007/s10327-004-0165-0
  15. Lecoq, H. and Pitrat, M. 1984. Strains of ZYMV in muskmelon (Cucumis melo L.) Phytopathol. Z. 111:165-173 https://doi.org/10.1111/j.1439-0434.1984.tb00488.x
  16. Lecoq, H. and Purcifull, D. E. 1992. Biological variability of potyviruses, an example: zucchini yellow mosaic virus. Arch. Virol. Suppl. 5:229-234 https://doi.org/10.1007/978-3-7091-6920-9_22
  17. Lee, K. C. and Wong, S. M. 1998. Variability of PI protein of zucchini yellow mosaic virus for strain differentiation and phylogenetic analysis with other potyviruses. DNA Seq. 9:275-293 https://doi.org/10.3109/10425179809008467
  18. Lee, S. C., Wu, M. and Wong, S. M. 1993. Nucleotide sequence of a Singapore isolate of zucchini yellow mosaic virus coat protein gene revealed an altered DAG motif. Virus Genes 7:381-387 https://doi.org/10.1007/BF01703393
  19. Lin, S. S., Hou, R. E and Yeh, S. D. 2000. Complete genome sequence and genetic organization of a Taiwan isolate of Zucchini yellow mosaic virus. Bot. Bull. Acad. Sin. 42:243-250
  20. Lisa, V., Boccardo, G., D'Agostino, G., Dellavalle, G. and D'Agostino, M. 1981. Characterization of a potyvirus that causes zucchini yellow mosaic. Phytopathology 71:667-672 https://doi.org/10.1094/Phyto-71-667
  21. Lisa, V. and Lecoq, H. 1984. Zucchini yellow mosaic virus. In: Murant, A. E and Harrison, B. D. (eds) CMI/AAB Description of plant viruses. AAB, UK
  22. Quemada, H., Sieu, L. C., Siemieniak, D. R., Gonsalves, D. and Slightom. J. L. 1990. Watermelon mosaic virus II and zucchini yellow mosaic virus: cloning of 3'-terminal regions, nucleotide sequences, and phylogenetic comparisons. J. Gen. Virol. 71:1451-1460 https://doi.org/10.1099/0022-1317-71-7-1451
  23. Pfosser, M. and Baumann, H. 2002. Phylogeny and geographical of Zucchini yellow mosaic virus isolates (Potyviridae) based on molecular analysis of the coat protein and part of the cytoplasmic inclusion protein genes. Arch. Virol. 147: 1599-1609 https://doi.org/10.1007/s00705-002-0839-x
  24. Provvidenti, R. Gonsalves, D. and Hurnaydan, H. S. 1984. Occurrence of zucchini yellow mosaic virus in cucurbits from Connecticut, New York, Florida, and California. Plant Dis. 68:443-446 https://doi.org/10.1094/PD-69-443
  25. Riechmann, J. L., Lain, S. and Garcia, J. A. 1992. Highlights and prospects of potyvirus molecular biology. J. Gen. Virol. 73: 1-16 https://doi.org/10.1099/0022-1317-73-1-1
  26. Siaw, M. F. E., Shahababuddin, M., Ballad, S., Shaw, J. G. and Roads, R. E. 1985. Identification of a protein covalently linked to the 5' terminus of Tobacco vein mottling virus RNA. Virology 142:134-143 https://doi.org/10.1016/0042-6822(85)90428-3
  27. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning. A laboratory Manual. Cold Spring Harbor, NY: Cold. Spring Harbor Laboratory
  28. Sanger, F., NikIen, S. and Colson, A. R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467
  29. Shukla, D. D., Lauricella, R. and Ward, C. W. 1988. The Nand C termini of the coat protein of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. J. Gen. Virol. 69: 1487-1508 https://doi.org/10.1099/0022-1317-69-7-1487
  30. van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L., Carsten, E. B., Esates, M. K., Lemon, S. M., Maniloff, J., Mayo, M. A., McGeoch, D. J., Pringles, C. R. and Widmer, R. B. 2000. VIrus Taxonomy: Classification and Nomenclature of Viruses: Seventh Report of the International Committee on Taxonomy of Viruses: San Diego, Academic press
  31. Yoon, J. Y. 1999. Constructionoffull-length cDNAs for genomic RNAs of Zucchini yellow mosaic potyvirus (ZYMV-Cu) iso-lated from cucumber. M.S. thesis. Kangwon National University
  32. Yoon, J. Y. and Choi, J. K. 1998. Nucleotide sequence of 3-terminal region of Zucchini yellow mosaic virus (cucumber isolate) RNA. Korean J. Plant Pathol. 14:23-27
  33. Zhao, M. F., Chen, J., Zheng, H. Y., Adams, M. J. and Chen, J. P. 2003. Molecular analysis of Zucchini yellow mosaic virus isolates from Hangzhou, China. J. Phytopathol. 151:307-311 https://doi.org/10.1046/j.1439-0434.2003.00724.x

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