Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibitory Effects of Acanthopanax chiisanensis Ethanolic Extracts on FcεRI α Chain Expression

지리 오갈피의 FcεRI α chain 발현 저해 효과

  • Shim, Sun-Yup (Oriental Medicine Research and Development Institute, Pukyong National University) ;
  • Sung, Chan-Ki (Department of Food Science, Jinju National University) ;
  • Lee, Sang-Won (Department of Microbiological Engineering, Jinju National University) ;
  • Choi, Young-Ju (Department of Food and Nutrition, Silla University) ;
  • Kim, Hyeung-Rak (Major of Nutrition, Pukyong National University) ;
  • Byun, Dae-Seok (major of Nutrition, Pukyong National University)
  • 심선엽 (부경대학교 한약재개발연구소) ;
  • 성찬기 (진주산업대학교 식품과학과) ;
  • 이상원 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 식품영양학과) ;
  • 김형락 (부경대학교 영양학과) ;
  • 변대석 (부경대학교 영양학과)
  • Published : 2007.11.30
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Abstract

Basophils and mast cells play an important role in $Fc{\varepsilon}RI-mediated$ allergic reaction as effector cells. We studied the effects of Acanthopanax chiisanensis on $Fc{\varepsilon}RI\;{\alpha}$ chain expression in human basophilic KU812F cells. Ethanol extracts from root and stem of A. chiisanensis were tested for inhibitory effects of $Fc{\varepsilon}RI\;{\alpha}$ chain expression. The cell surface $Fc{\varepsilon}RI\;{\alpha}$ chain expression was examined by flow cytometric analysis. All of the extracts of A. chiisanensis reduced the cell surface $Fc{\varepsilon}RI\;{\alpha}$ chain expression. Furthermore, A. chiisanensis extracts caused a decrease in the level of $Fc{\varepsilon}RI\;{\alpha}$ chain mRNA level and $Fc{\varepsilon}RI-mediated$ histamine release. These results suggest that root and stem extracts of A. chiisanensis play an important role in anti-allergic activity via down-regulation of $Fc{\varepsilon}RI\;{\alpha}$ chain expression and decrease in release of inflammatory mediator such as histamine.

호염기구와 비만세포는 $Fc{\varepsilon}RI$을 매개로 한 알러지 반응에 있어 효과세포로서 중요한 역할을 담당한다. 인간 유래 호염기구성 세포주 KU812F 세포의 $Fc{\varepsilon}RI\;{\alpha}$ chain 발현에 있어 지리 오갈피의 저해 효과에 대해 연구하였다. 지리 오갈피의 뿌리 및 줄기를 에탄올로 추출하여, $Fc{\varepsilon}RI\;{\alpha}$ chain 저해 활성 실험에 이용하였다. 세포 표면의 $Fc{\varepsilon}RI\;{\alpha}$ chain 발현량을 flow cytometry로 분석한 결과, 지리 오갈피 뿌리 및 줄기 추출물에서 세포표면의 $Fc{\varepsilon}RI$ 발현을 억제하는 효과를 나타냈다. 또한 지리 오갈피 뿌리 및 줄기 추출물은 $Fc{\varepsilon}RI\;{\alpha}$ chain mRNA 발현을 감소시켰으며, $Fc{\varepsilon}RI$을 매개로 한 히스타민 유리를 감소시켰다. 이러한 결과는 지리 오갈피의 뿌리 및 줄기 추출물이 $Fc{\varepsilon}RI\;{\alpha}$ chain 발현의 저하 조절 및 히스타민과 같은 염증매개인자의 분비를 저하시킴으로서 항 알러지 활성을 갖는데 중요한 역할을 할 것으로 판단된다.

Keywords

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