생명과학회지 (Journal of Life Science)

  • 제17권10호
  • /
  • Pages.1381-1386
  • /
  • 2007
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

열처리에 의한 황금(Scutellaria baicalensis) 추출물의 생리활성 변화

Changes in Physiological Activities of Scutellariae baicalensis by Heating

  • 최명락 (전남대학교 생명산업공학) ;
  • 이진승 (전남대학교 생명산업공학) ;
  • 임현수 (전남대학교 생명산업공학)
  • Choi, Myeong-Rak (Department of Biotechnology, Chonnam National University) ;
  • Lee, Jin-Seung (Department of Biotechnology, Chonnam National University) ;
  • Lim, Hyun-Soo (Department of Biotechnology, Chonnam National University)
  • 발행 : 2007.10.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

황금에 대한 수 추출물의 열 변환에 의한 생리활성 변화를 보기 위하여 HPLC를 통한 peak분석과 기능성 평가를 하였다. 열처리 전의 시료 (CW)와 열처리 후의 시료 (CHW)간의 peak분석에서 CW와 CHW의 비교 시 A와 B가 없어지고 열처리 후에 C라는 다른 물질의 peak가 형성됨을 알 수 있었다. 또한, 전체적인 기능성 평가에서 항산화성에서는 CW와 CHW 별 차이가 없었고, 항고혈압 활성은 CW가 높았다. 항암성에 있어서 폐암에서는 CW가 높았고, 위암, 간암, 후두암 등에는 CHW가 높게 나왔다. 그리고, 멜라닌 생성세포의 멜라닌 생성 억제는 CHW에서 약 80%로 매우 높게 나타났다. 결론적으로 열처리를 통한 시료 내 구성성분의 변환이 기능성 차이를 보여주며, 이는 새로운 열 변환 물질의 생성 때문으로 판단된다. 이들 변화된 물질은 황금의 주성분인 Baicalin 외의 물질이 열 변환 된 것으로, 생성된 물질에 대한 구조분석이 필요한 것으로 사료된다.

To elucidate the changes in physiological activities of Scutellariae baicalensis by heating, composition analysis with HPLC and evaluation of physiological activities of extracts were conducted. HPLC analysis of CW(water extract at room temperature) and CHW(heat treated CW) indicated that two distinctive peaks in the HPLC chromatogram were disappeared after boiling and additional peak was newly observed. This observation suggested that certain chemical changed in the extract occurred by heat treatment. In the estimation of physiological activities, CW showed higher ACE inhibiting activity than CHW. In the case of cytotoxic activity against cancer cells, CW exhibited higher activity than CHW against CCL-185. On the other hand, CHW showed higher activity than CW against HeLa, SNU-601 and HepG2. In addition, CHW showed the inhibitory effect on melanogenesis (80% inhibition) while CW did not show any activity.

키워드

참고문헌

  1. Ando, S., O. Ando, Y. Suemoto and Y. Mishima. 1993. Tyrosinase gene transcription and its control by melanogenetic inhibitor. J. Invest Dermatol. 100, 150-155. https://doi.org/10.1111/1523-1747.ep12465056
  2. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1121.
  3. Cha, J. Y., S. H. Park, J. S. Heo and Y. S. Cho. 2007. Effects of water extract from fermented Chaga mushroom( Inonotus obliquus) on the proliferation of human cancer cell lines. J. Life Sci. 17, 671-677. https://doi.org/10.5352/JLS.2007.17.5.671
  4. Cho, S. H. and Y. R. Kim. 2001. Antimicrobial effects of Scutellariae radix extract against Listeria monocytogenes. J. Korean Soc. Food Sci. Nutr. 30, 959-963.
  5. Cushman, D. W. and H. S. Cheung. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochemical Pharmacology 20, 1637-1648. https://doi.org/10.1016/0006-2952(71)90292-9
  6. Hashiguchi, H. and H. Takahashi. 1976. Inhibition of two copper containing enzymes, tyrosinase and dopamin $\beta$- hydroxylase by L-Mimosiine. Molecular Pharmacology 13, 362-367.
  7. Hwang, S. W., J. Lee, H. S. Kwon, K. D. Lee, S. H. Nam, K. H. Park and M. S. Yang. 2005. Comparison of tyrosinase inhibitory effect of the natural antioxidants from Cedrela sinensis. Agric. Chem. Biotechnol. 48, 144-147.
  8. Imokawa, G. and Y. Mishima. 1982. Loss of melanogenic properties in tyrosinase induced by glycosilation inhibitors within malignant melanoma cells. Cancer Res. 42, 1994-2002.
  9. Ishihara, K., T. Takemura, Y. Hamada, C. Sakai, S. Kondoh, S. Nishiyama and K. Urabe. 1993. Pigment production in murine melanoma cell is regulated by tyrosinase, tyrosinase-related protein (TRP1), DOPAchrome tautomerase (TRP2) and a melanogenic inhibitor. J. Invest Dermatol. 2, 126-131.
  10. Kim, Y. R. and S. H. Cho. 2002. Antimicrobial activities of Scutellariae radix extract against Vibrio parahaemolyticus. J. Korean Soc. Food Sci. Nutr. 31, 534-538. https://doi.org/10.3746/jkfn.2002.31.3.534
  11. Kwak, J. H., Y. H. Kim, H. R. Chang, C. W. Park and Y. H. Han. 2004. Inhibitory effect of gardenia fruit extracts on tyrosinase activity and melanogenesis. Korean J. Biotechnol. Bioeng. 19, 437-440.
  12. Lee, B. C., K. H. Leem, Y. O. Kim, S. Y. Kim, D. K. Ahn, H. K. Park and H. C. Kim. 1999. Neuroprotective effects of Scutellariae radix on the brain ischemia induced by four-vessel occlusion in rats. Korean J. Herbology 14, 89-96.
  13. Lim, S. Y., K. H. Kim, K. Y. Park and S. H. Lee. 2007. Effect of solvent fractions from Doenjang on antimutagenicity, growth of tumor cells and production of Interleukin-2. J. Life Sci. 17, 791-797. https://doi.org/10.5352/JLS.2007.17.6.791
  14. Lim, S. Y., K. Y. Park, S. H. Lee and J. S. Choi. 2007. Inhibitory effect of methanol extracts and solvent fractions from Meju on mutagenicity and growth of human cancer cells. J. Life Sci. 17, 76-81. https://doi.org/10.5352/JLS.2007.17.1.076
  15. Lopez, J. N. R., J. Tudela, R. Varon, F. G. Carmona and F. G. Canovas. 1992. Analysis of a kinetic model for melanin biosynthesis pathway. J. Biol. Chem. 267, 3901-3910.
  16. Masuda, M., T. Tajima and T. Suzuki. 1996. Skin lighteners, Cosmetics & Toiletries 111, 65-77.
  17. Oh, S. S., E. J. Seo, H. Y. Kim, Y. B. Ryu, J. H. Lee, S. W. Gal and K. H. Park. 2007. Tyrosinase inhibitory Xanthones from Cudrania tricuspidata. J. Life Sci. 17, 476-481. https://doi.org/10.5352/JLS.2007.17.4.476
  18. Park, M. K., K. S. Jung and M. J. In. 2004. Effects of Scutellaria baicalensis and Phellodendron amurensis extracts on growth of lactic acid bacteria and Kimchi fermentation. J. Korean Soc. Food Sci. Nutr. 33, 420-426. https://doi.org/10.3746/jkfn.2004.33.2.420
  19. Park, S. S., Y. B. Ryu, Y. H. Lee, Y. U. Cho, S. J. Cho, Y. J. Choi, K. H. Park and S. W. Gal. 2007. Inhibition of melanin synthesis by mycelial culture broth of Paecilomyces japonica in the mulberry leaf extract. J. Life Sci. 17, 816-821. https://doi.org/10.5352/JLS.2007.17.6.816
  20. Rhee, J. S., E. R. Woo, N. H. Kim, E. J. Lee, D. K. An, J. H. Lee, S. K. Park and H. K. Park. 1997. A study on qualitative and quantitative analysis of major ingredients in Scutellariae radix. Analytical Sci. & Technol. 10, 91-104.
  21. Yang, J. H., D. S. Kim, H. D. Yoo and N. H. Lee. 1996. Preparation and bioavailability of Oriental Medicine containing Baicalin (II). J. Korean Pharm. Sci. 26, 91-98.

피인용 문헌

  1. Antibacterial Activity of Scutellaria baicalensis Extract against Antibiotic Resistant Bacteria vol.24, pp.4, 2011, https://doi.org/10.9799/ksfan.2011.24.4.708
  2. Production of Baicalin, Baicalein, and Wogonin on Plant Tissue Culture of Scutellaria baicalensis vol.28, pp.4, 2015, https://doi.org/10.7732/kjpr.2015.28.4.526