Journal of Plant Biotechnology

  • 제34권3호
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  • Pages.271-275
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  • 2007
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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적은 수의 거대 엽록체를 가진 핵 형질전환 식물체를 이용한 담배 엽록체 형질전환 빈도 제고

Enhancement of Chloroplast Transformation Frequency by Using Mesophyll Cells Containing a Few Enlarged Chloroplasts from Nuclear Transformed Plants in Tobacco

  • 정원중 (한국생명공학연구원 식물유전체연구센터) ;
  • 민성란 (한국생명공학연구원 식물유전체연구센터) ;
  • 유장렬 (한국생명공학연구원 식물유전체연구센터)
  • Jeong, Won-Joong (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Min, Sung-Ran (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Liu, Jang-R. (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 발행 : 2007.09.29
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초록

엽록체 형질전환된 식물체를 얻으려면 먼저 세포수준에서 모든 엽록체가 형질전환되어야 하는데, 세포내에는 많은 수의 엽록체가 존재하므로, 엽록체 형질전환 벡터가 전이되어 형질전환된 엽록체는 선발배지에서 선택적으로 분열을 계속하고 형질전환되지 않은 엽록체들은 분열을 하지 못하게 되어, 결국 해당 세포내의 모든 엽록체가 형질전환된 상태에 이르게 된다. 따라서 만일 해당 세포내에 엽록체의 수가 적으면 그만큼 효율적으로 엽록체 형질전환을 할 수 있을 것이다. 본 연구에서는 담배의 FtsZ 유전자를 핵형질전환법으로 과잉 발현시킴으로써 엽록체의 분열이 저해되어 엽육세포내에 거대한 엽록체 3-5개를 가진 담배식물체의 엽육조직을 이용하여, 엽록체 형질전환을 한 결과, 엽록체 형질전환 빈도가 약 40% 증가되었다.

In the chloroplast transformation process, a chloroplast containing transformed chloroplast genome copies should be selected over wild-type chloroplasts on selection medium. It is more effective for a cell to become homoplasmic if the cell contains smaller number of chloroplasts. Therefore, to reduce the number of chloroplasts in mesophyll cells in tobacco, we overexpressed FtsZ to generate transgenic plants, of which mesophyll cell contained a few enlarged chloroplasts contrast to a wild-type mesophyll cell containing approximately 100 chloroplasts. It was demonstrated that transgenic leaf tissues comprising cells with a few enlarged chloroplasts gave rise to approximately 40% higher frequency of chloroplast-transformed adventitious shoots.

키워드

참고문헌

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피인용 문헌

  1. Genetic Discrimination of Catharanthus roseus Cultivars by Pyrolysis Mass Spectrometry vol.52, pp.5, 2009, https://doi.org/10.1007/s12374-009-9059-1
  2. Production of stable chloroplast-transformed plants in potato (Solanum tuberosum L.) vol.38, pp.1, 2011, https://doi.org/10.5010/JPB.2011.38.1.042