Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
권호 표지
DOI QR코드

DOI QR Code

QCM biosensor for the determination of haptoglobin

헵토글로빈 측정을 위한 수정미소저울 바이오센서 개발

  • Published : 2007.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose this research is to develop QCM (quartz crystal microbalance) biosensor for the determination of haptoglobin. Haptoglobin is an acute-phase protein with a hemoglobin-binding activity and has a potential to be used as a biomarker for infection or cancer. Haptoglobin level in milk has been used for the diagnosis of cow mastitis. In this study, anti-bovine haptoglobin antibody or bovine hemoglobin was chemically immobilized on the surface of the QCM, and the resulting sensor chips were tested for their response to samples containing haptoglobin at different concentrations. Concentration dependent frequency change was observed with both of the sensor chips. Especially, the sensor chip containing anti-bovine haptoglobin antibody showed sufficient sensitivity in the concentrations typically observed in the cows with mastitis.

Keywords

References

  1. H. H. Petersen, J. P. Nielsen, and P. M. Heegaard, 'Application of acute phase protein measurements in veterinary clinical chemistry', Vet. Res., vol. 35, pp. 163-187, 2004 https://doi.org/10.1051/vetres:2004002
  2. U. Gronlund, C. H. Sandgren, and K. P. Waller, 'Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis', Vet. Res., vol. 36, pp. 191-198, 2005 https://doi.org/10.1051/vetres:2004063
  3. H. Van Vlierberghea, M. Langloisb, and J. Delanghe, 'Haptoglobin polymorphisms and iron homeostasis in health and in disease', Clin. Chim. Acta, vol. 345, pp. 35-42, 2004 https://doi.org/10.1016/j.cccn.2004.03.016
  4. M. Kotaka, G. G. Chen, P. B. Lai, W. Y. Lau, P. K. Chan, T. W. Leung, and A. K. Li, 'Analysis of differentially expressed genes in hepatocellular carcinoma with hepatitis C virus by suppression subtractive hybridization', Oncol. Res., vol. 13, pp. 161-167, 2002
  5. Y. Kaneta, Y. Kagami, T. Tsunoda, R. Ohno, Y. Nakamura, and T. Katagiri, 'Genome-wide analysis of gene-expression profiles in chronic myeloid leukemia cells using a cDNA microarray', Int. J. Oncol., vol. 23, pp. 681-691, 2003
  6. B. Ye, D. W. Cramer, S. J. Skates, S. P. Gygi, V. Pratomo, L. Fu, N. K. Horick, L. J. Licklider, J. O. Schorge, R. S. Berkowitz, and S. C. Mok, 'Haptoglobin-alpha subunit as potential serum biomarker in ovarian cancer: identification and characterization using proteomic profiling and mass spectrometry', Clin. Cancer. Res., vol. 9, pp. 2904-2911, 2003
  7. H. F. Juan, J. H. Chen, W. T. Hsu, S. C. Huang, S. T. Chen, J. Y.-C. Lin, Y. W. Chang, C. Y. Chiang, L. L. Wen, D. C. Chan, Y. C. Liu, and Y. J. Chen, 'Identification of tumor-associated plasma biomarkers using proteomic techniques: From mouse to human', Proteomies, vol. 4, pp. 2766-2775, 2004 https://doi.org/10.1002/pmic.200400785
  8. M. Akerstedt, L. Bjorck, K. P. Waller, and A. Stemesjo, 'Biosensor assay for determination of haptoglobin in bovine milk', J. Daily Res., vol. 73, pp. 299-305, 2006
  9. A. J. Eun, L. Huang, F. T. Chew, S. F. Li, and S. M. Wong, 'Detection of two orchid viruses using quartz crystal microbalance (QCM) immunosensors', J. Virol. Methods, vol. 99, pp. 71-79, 2002 https://doi.org/10.1016/S0166-0934(01)00382-2
  10. Y. S. Fung and Y. Y. Wong, 'Self-assembled monolayers as the coating in a quartz piezoelectric crystal immunosensor to detect Salmonella in aqueous solution', Anal. Chem., vol. 73, pp. 5302-5309, 2001 https://doi.org/10.1021/ac010655y
  11. X. L. Su and Y. Li, 'A self-assembled monolayerbased piezoelectric immunosensor for rapid detection of Escherichia coli 0157:H7', Biosens. Bioelectron., vol. 19, pp. 563-574, 2004 https://doi.org/10.1016/S0956-5663(03)00254-9
  12. L. Pastorino, F. Caneva Soumetz, M. Giacomini, and C. Ruggiero, 'Development of a piezoelectric immunosensor for the measurement of paclitaxel', J. lmmunol. Methods, vol. 313, pp. 191-198, 2006 https://doi.org/10.1016/j.jim.2006.05.003
  13. S. F. Chou, W. L. Hsu, J. M. Hwang, and C. Y. Chen, 'Determination of alpha-fetoprotein in human serum by a quartz crystal microbalance-based immunosensor', Clin. Chem., vol. 48, pp. 913-918, 2002
  14. S. Y. Kwon, J. C. Kim, and B. I. Choi, 'Development of QCM dew point sensor and its sensing characteristics study', J. of the Korean Sensors Society, vol. 15, pp. 269-276, 2006 https://doi.org/10.5369/JSST.2006.15.4.269
  15. M. M. Vareiro, J. Liu, W. Knoll, K. Zak, D. Williams, and A. T. Jenkins, 'Surface plasmon fluorescence measurements of human chorionic gonadotrophin: role of antibody orientation in obtaining enhanced sensitivity and limit of detection', Anal. Chem., vol. 77, pp. 2426-2431, 2005 https://doi.org/10.1021/ac0482460
  16. V. B. Kandimalla, N. S. Neeta, N. G. Karanth, M. S. Thakur, K. R. Roshini, B. E. Rani, A. Pasha, and N. G. Karanth, 'Regeneration of ethyl parathion antibodies for repeated use in immunosensor: a study on dissociation of antigens from antibodies', Biosens. Bioelectron., vol. 20, pp. 903-906, 2004 https://doi.org/10.1016/j.bios.2004.03.027
  17. X. Su, F. T. Chew, and S. F. Li. 'Self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of total human immunoglobulin E', Anal. Biochem., vol. 273, pp. 66-72, 1999 https://doi.org/10.1006/abio.1999.4186
  18. N. K. Chaki and K. Vijayamohanan, 'Self-assembled mono layers as a tunable platform for biosensor applications', Biosens. Bioelectron., vol. 17, pp. 112, 2002
  19. C. Kumar, Nanosystem Characterization Tools in the Life Sciences, Wiley-VCH, Weinheim, Germany, pp. 109-144, 2006
  20. T. Okazaki, Y. Yanagisawa, and T. Nagai, 'Analysis of the affinity of each haptoglobin polymer for hemoglobin by two-dimensional affinity electrophoresis', Clin. Chim. Acta., vol. 258, pp. 137-144, 1997 https://doi.org/10.1016/S0009-8981(96)06468-6
  21. P. D. Eckersall and J. G. Conner, 'Plasma haptoglobin in cattle (Bos taurus) exists as polymers in association with albumin', Comp. Biochem. Physiol. B, vol. 96, pp. 309-314, 1990 https://doi.org/10.1016/0305-0491(90)90379-8
  22. M. Morimatsu, B. Syuto, N. Shimada, T. Fujinaga, S. Yamamoto, M. Saito, and M. Naiki, 'Isolation and characterization of bovine haptoglobin from acute phase sera', J. BioI. Chem., vol. 266, pp. 11833-11837, 1991
  23. S. C. Tam and J. T. Wong, 'Modification of hemoglobin upon covalent coupling to dextran: enhanced stability against acid denaturation and reduced affinity for haptoglobin', Can. J. Biochem., vol. 58, pp. 732-736, 1980 https://doi.org/10.1139/o80-103