Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functional Dissection of Sigma-like Domain in Antibiotic Regulatory Gene, afsR2 in Streptomyces lividans

  • Kim Chang-Young (School of Chemical Engineering and Biotechnology, Department of Biological Engineering, Inha University) ;
  • Park Hyun-Joo (School of Chemical Engineering and Biotechnology, Department of Biological Engineering, Inha University) ;
  • Kim Eung-Soo (School of Chemical Engineering and Biotechnology, Department of Biological Engineering, Inha University)
  • Published : 2006.09.01
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Abstract

The 63-amino-acid-encoding afsR2 is a global antibiotics-stimulating regulatory gene identified from the chromosome of Streptomyces lividans. To dissect a putative functional domain in afsR2, several afsR2-derivative deletion constructs were generated and screened for the loss of actinorhodin-stimulating capability. The afsR2-derivative construct missing a 50-bp C-terminal region significantly lost its actinorhodin-stimulating capability in S. lividans. In addition, site-directed mutagenesis on amino acid positions of #57-#61 in a 50-bp C-terminal region, some of which are conserved among known Sigma 70 family proteins, significantly changed the AfsR2's activity. These results imply that the C-terminal region of AfsR2 is functionally important for antibiotics-stimulating capability and the regulatory mechanism might be somehow related to the sigma-like domain present in the C-terminal of AfsR2.

Keywords

References

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