Journal of Microbiology and Biotechnology

  • 제16권9호
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  • Pages.1472-1476
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Gene Disruption Using In Vivo and In Vitro Methylation in Streptomyces griseus

  • Maeng Jin-Soo (Laboratory of Biophysical Chemistry, National Heart, Lung and Blood Institute, National Institutes of Health) ;
  • Bae Kyung-Sook (Laboratory of Insect Resources, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwak Jang-Yul (Laboratory of Insect Resources, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2006.09.01
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초록

Previous study demonstrated that the restriction barrier of Streptomyces griseus is almost completely bypassed by the Streptomyces-E. coli shuttle vectors passed through the E. coli GM161 strain and methylated with AluI and HpaII methyltransferases. The same DNA methylation of the genomic DNA fragments cloned the nonreplicative vectors generated integrative transformation and gene disruption of their chromosomal counterparts at high efficiencies in S. griseus. This result indicated that the efficiency of gene disruption depends on the efficient transfer of the incoming DNA into bacterial hosts.

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참고문헌

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