Journal of Microbiology and Biotechnology

  • 제16권9호
  • /
  • Pages.1429-1433
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Expression and Purification of a Cathelicidin-Derived Antimicrobial Peptide, CRAMP

  • Park Eu-Jin (Department of Advanced Fusion Technology and Bio/Molecular Informatics Center, Konkuk University) ;
  • Chae Young-Kee (Department of Chemistry and Recombinant Protein Expression Center, Sejong University) ;
  • Lee Jee-Young (Department of Advanced Fusion Technology and Bio/Molecular Informatics Center, Konkuk University) ;
  • Lee Byoung-Jae (Laboratory of Molecular Genetics, School of Biological Sciences, Institute of Molecular Biology and Genetics) ;
  • Kim Yang-Mee (Department of Advanced Fusion Technology and Bio/Molecular Informatics Center, Konkuk University)
  • 발행 : 2006.09.01
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초록

Application of recombinant protein production and particularly their isotopic enrichment has stimulated development of a range of novel multidimensional heteronuclear NMR techniques. Peptides in most cases are amenable to assignment and structure determination without the need for isotopic labeling. However, there are many cases where the availability of $^{15}N$ and/or $^{13}C$ labeled peptides is useful to study the structure of peptides with more than 30 residues and the interaction between peptides and membrane. CRAMP (Cathelicidin-Related AntiMicrobial Peptide) was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. CRAMP was successfully expressed as a GST-fused form in E. coli and purified using affinity chromatography and reverse-phase chromatography. The yield of the CRAMP was 1.5 mg/l 1. According to CD spectra, CRAMP adopted ${\alpha}$-helical conformation in membrane-mimetic environments. Isotope labeling of CRAMP is expected to make it possible to study the structure and dynamic properties of CRAMP in various membrane systems.

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