Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immunogenicity of Recombinant Outer Membrane Protein H from Pasteurella multocida

재조합 파스튜렐라 외막 단백질 H의 면역원성 검정

  • Lee Jeong-Min (Institute of Life Science and Technology, Sungkyunkwan University)
  • 이정민 (성균관대학교 생명공학연구소)
  • Published : 2006.09.01
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Abstract

To investigate the antigenicity and protective immunity of outer membrane protein H (OmpH) in Pasteurella multocida D:4, the recombinant OmpH protein was produced in Escherichia coli. The truncated and Trx-fused form of recombinant OmpH (53 kDa) was purified, and used as an antigen in the immunization and challenge experiment. The immunized mice with the recombinant OmpH produced a high-titer antibody, and had protective immunity against P. multocida as same level as the mice immunized with formalin-killed whole cell.

본 연구에서는 병원성 Pasteurella multocida D:4 외막 단백질 H의 방어적 면역성과 백신으로서의 가능성을 검정하고자, 외막 단백질 H 유전자를 대장균에서 발현, Trx와 융합된 형태의 재조합 외막 단백질 H를 분리하여 면역화와 백신 실험에 항원으로 사용하였다. 면역 실험에서 재조합 외막 단백질 H는 높은 역가의 항체를 유도하였으며, 불활화한 사균 백신과 유사한 수준의 백신 효과를 나타내었다.

Keywords

References

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