Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimal Production and Characterization of Laccase from Fomitella fraxinea Mycelia

Fomitella fraxinea 균사체로부터 Laccase의 최적생산 및 효소적 특성

  • 박경미 (동국대학교 과학기술대학 생명공학과) ;
  • 박상신 (동국대학교 과학기술대학 생명공학과)
  • Published : 2006.09.01
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Abstract

The culture conditions were investigated to maximize the production of laccase from Fomitella fraxinea mycelia. Among the tested media, mushroom complete medium (MCM) showed the highest production of the enzyme. The optimum culture medium was 2% dextrose, 0.4% $(NH_4)_{2}HPO_4$, 0.05% $Na_{2}HPO_{4}{\cdot}7H_{2}O$, and 0.05% KCl as carbon, nitrogen, phosphorus, and inorganic salt sources respectively. SDS-PAGE followed by laccase activity staining using 2,6-djmethoxyphenol as the substrate was performed to identify the laccase activity under culture conditions studied. Zymogram analysis of the culture supernatant showed a laccase band with a molecular mass of 50 kDa. The enzyme production from F. fraxinea was reached to the highest level after the cultivation for 10 days at $25^{\circ}C$ and initial pH 8. The enzyme activity of the culture supernatant was most active at $50^{\circ}C$ and pH 5.

장수버섯(Fomitella fraxinea)의 균사체 배양액으로부터 laccase를 생산하기 위한 최적 배양조건을 조사하였다. 복합 배지 중에서 MCM이 laccase의 생산에 가장 우수하였으며, laccase를 생산하기 위한 최적 배지조건은 탄소원, 질소원, 인산원 및 무기질원으로 각각 2% dextrose, 0.4% $(NH_4)_{2}HPO_4$, 0.05% $Na_{2}HPO_{4}{\cdot}7H_{2}O$ 및 0.05% KCl의 첨가에 의하여 효소의 생산이 가장 높았다. 따라서 E. fraxinea로 부터 laccase를 생산하기 위한 최적 배지조건은 2% dextrose, 0.4% $(NH_4)_{2}HPO_4$, 0.05% $Na_{2}HPO_{4}{\cdot}7H_{2}O$ 및 0.05% KCl 이다. 이상의 배지를 사용하여 배양온도 $25^{\circ}C$, 초기 pH 8.0에서 10일 동안 배양하였을 때 효소의 생산이 가장 증가함을 알 수 있었다. Staib agar plate 상에서 장수 버섯(Fomitella fraxinea) 균사체의 laccase활성여부를 확인할 수 있었으며 배양액 중의 효소 활성의 최적 pH와 온도는 pH 5.0과 $50^{\circ}C$ 이었다.

Keywords

References

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