Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Early Exposure to Anti-androgen Compounds Induces the Delay in the Testis Development in Immature Male Rat

항안드로겐성 물질이 성 성숙 이전 단계의 정소에서 미치는 영향 연구

  • Hong Jin (National Institute of Toxicological Research Endocrine Toxicology Division) ;
  • Han Soon-Young (National Institute of Toxicological Research Endocrine Toxicology Division) ;
  • Moon Hyun-Ju (National Institute of Toxicological Research Endocrine Toxicology Division) ;
  • Kang Tae-Seok (National Institute of Toxicological Research Endocrine Toxicology Division) ;
  • Kang Il-Hyun (National Institute of Toxicological Research Endocrine Toxicology Division) ;
  • Kim Tae-Sung (National Institute of Toxicological Research Endocrine Toxicology Division) ;
  • Kim Seung-Hee (National Institute of Toxicological Research Endocrine Toxicology Division) ;
  • Kwon Ki-Sung (National Institute of Toxicological Research Endocrine Toxicology Division)
  • 홍진 (국립독성연구원 내분비장애물질팀) ;
  • 한순영 (국립독성연구원 내분비장애물질팀) ;
  • 문현주 (국립독성연구원 내분비장애물질팀) ;
  • 강태석 (국립독성연구원 내분비장애물질팀) ;
  • 강일현 (국립독성연구원 내분비장애물질팀) ;
  • 김태성 (국립독성연구원 내분비장애물질팀) ;
  • 김승희 (국립독성연구원 내분비장애물질팀) ;
  • 권기성 (국립독성연구원 내분비장애물질팀)
  • Published : 2006.09.01
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Abstract

The experiments investigated whether early exposure to testosterone propionate (TP) during prepuberty alters testis development in Sprague-Dawley male rats. We performed Hershberger assay using the stimulated weanling male rats by OECD protocols, cDNA microarray, and Western blot. TP was subcutaneously injected to uncastrated Sprague-Dawley male rat of 22 days old for 10 consecutive days at doses of 0.4, 0.8, 1.0, 1.2, 1.6 mg/kg per day. At necropsy, the following tissues were removed and weighed: combined testes, epididymides (Epi), Cowper's glands (COW), levator am, and bulbocavernosus muscles (LABC), seminal vesicles, together with coagulating gland (SV) and ventral prostate (VP). We found that TP increased the weights of Epi, VP, SV, COW, and LABC, while testis was decreased in a dose-dependent manner. In cDNA microarray analysis of testis, there were significant reductions in the expression of cytochrome P450 11A (CYP11A), the rate-limiting enzyme of steroidogenesis. Taken together these results, TP exposure before puberty in male rats may produce the delay in testis development by inhibiting the CYP11A gene expression.

Keywords

References

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