Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Evaluation of the Genetic Toxicity of Synthetic Chemicals (XV) -In vivo Peripheral Blood Reticulocytes Micronucleus Assay of 17 Synthetic Chemicals in Mice-

합성화학물질들의 유전독성평가(XV) -마우스의 말초혈의 망상적혈구를 이용한 17종 합성화학물질들의 생체내 소핵시험-

  • Kim Mi-Soon (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Kim Youn-Jung (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Ryu Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology)
  • 김미순 (한국과학기술연구원, 분자 세포 독성 연구실) ;
  • 김연정 (한국과학기술연구원, 분자 세포 독성 연구실) ;
  • 류재천 (한국과학기술연구원, 분자 세포 독성 연구실)
  • Published : 2006.09.01
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Abstract

합성화학물질들이 환경으로의 유입은 인체에는 물론 환경생태계에 많은 영향을 미치므로 이들의 유해성 검증은 매우 중요한 일이라 할 수 있다. 실제 산업체에서 사용되는 수많은 화학물질들의 유전적 손상 유발유무는 유해성검증에서 무엇보다 중요한 일이라 할 수 있다. 이에 산업체 공정과정에서 널리 사용되는 것으로 알려진 17종의 합성화학물질에 대해 마우스의 말초 혈의 망상적혈구를 이용한 in vivo 소핵시험을 수행하여, 소핵형성 유발유무를 관찰하였다. 양성대조군으로 사용된 mitomycin C는 음성대조군과 비교시 유의하게 소핵을 유발하는 반면, 비교적 마우스에서 높은 50% 치사량을 보이는 benzoyl chloride, p-toluene sulfonic acid 및 4,4'-sulfonyldianiline 등의 합성물질들을 포함한 총 17종의 물질들은 본 실험결과 통계적으로 유의하게 소핵을 유발하지 않는 것을 관찰 할 수 있었다.

Keywords

References

  1. Ames BN, McCann J and Yamasaki E. Method for detecting carcinogens and mutagens with Salmonella/mammalian-microsome mutagenicity test, Mutat Res 1975; 31: 347-364 https://doi.org/10.1016/0165-1161(75)90046-1
  2. Becker P, Scherthan H and Zankl H. Use of a centromerespecific DNA probe (p82H) in nonisotopic in situ hybridization for classification of micronuclei, Genes Chromosomes Cancer 1990; 2: 59-62 https://doi.org/10.1002/gcc.2870020111
  3. Clive D, Bolcsfoldi G, Clements J, Cole J, Homna Masamitsu, Majeska J, Moore M, Muller L, Myhr B, Oberly T, Oudelhkim M-C, Rudd C, Shimada H, Sofuni T, Thybaud V and Wilcox P. Consensus agreement regarding protocol issues discussed during the mouse lymphoma workshop, Environ Mol Mutagenesis 1995; 25: 165-168 https://doi.org/10.1002/em.2850250211
  4. CSGMT (Collaborative Study Group for the Micronucleus Test). Micronucleus test with mouse peripheral blood erythrocytes by acridine orange supravital staining: the summary report of the 5th collaborative study by CSGMT/ JEMSMMS, Mutat Res 1992; 278: 83-98 https://doi.org/10.1016/0165-1218(92)90215-L
  5. Garriott ML, Casciano DA, Schechtman LM and Probst GS. International workshop on mouse lymphoma assay testing practices and data interpretations, Environ. Mol. Mutagenesis 1995; 25: 162-164 https://doi.org/10.1002/em.2850250210
  6. Hayashi M, Sofuni T and Ishidate M. Jr. An application of acridine orange fluorescent staining to the micronucleus test, Mutat Res 1983; 120: 241-247 https://doi.org/10.1016/0165-7992(83)90096-9
  7. Hayashi M, Morita T, Kodama Y, Sofuni T and Ishidate M Jr. The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides, Mutat Res 1990; 245: 245-249 https://doi.org/10.1016/0165-7992(90)90153-B
  8. Hayashi M. The Micronucleus Test, Scientist Tokyo 1991
  9. Hayashi M, Kodama Y, Awog T, Suzuki T, Asita AO and Sofuni T. The micronucleus assay using peripheral blood reticulocytes from mitomycin C- and cyclophosphamide-treated rats, Mutat Res 1992; 278: 209-213 https://doi.org/10.1016/0165-1218(92)90236-S
  10. Hayashi M, Ueda T, Uyeno K, Wada K, Kinae N, Saotome K, Tanaka N, Akai A, Sasaki YF, Asano N, Sofuni T and Ojima Y. Development of genotoxicity assay systems that use aquatic organisms, Mutat Res 1998; 399: 125-133 https://doi.org/10.1016/S0027-5107(97)00251-0
  11. Heddle JA. A rapid in vivo test for chromosomal damage, Mutat Res 1973; 18: 187-190 https://doi.org/10.1016/0027-5107(73)90035-3
  12. Heo MY, Kim JH and Ryu JC. Anticlastogenicity of betacarotene and galangin using in vivo supravital staining micronucleus test. Environ Mutagens & Carcinogens 1997; 17: 92-96
  13. IARC Summary & Evaluation, Vol. 71, 1999
  14. Ishidate M and Odashima S. Chromosome test with 134 compounds on chinese hamster cells in vitro-A screening for chemical carcinogens. Mutat Res 1977; 48: 337-354 https://doi.org/10.1016/0027-5107(77)90177-4
  15. Komae N, Hibino Y and Sugano N. Analysis of micronuclei induced under hyperthermic conditions in human lymphocyte culture by fluorescence in situ hybridization (FISH) and spectral karyotyping (SKY) methods, Yakugaku Zasshi 1999; 119: 763-772 https://doi.org/10.1248/yakushi1947.119.10_763
  16. Lorke D. A new approach to practical acute toxicity testing, Arch. Toxicol 1983; 54: 275-287 https://doi.org/10.1007/BF01234480
  17. MacGregor JT, Wehr CM, Henika PR and Shelby MD. The in vivo erythrocyte micronucleus test: measurement at steady state increases assay efficiency and permits integration with toxicity studies, Fundam. Appl Toxicol 1990; 14(3): 513-22 https://doi.org/10.1016/0272-0590(90)90255-I
  18. Maron DM and Ames BN. Revised methods for the Salmonella mutagenicity test, Mutat Res 1983; 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  19. Mavournin KH, Blakey DH, Cimino MC, Salamone MF and Heddle JA. The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the US Environmental Protection Agency Gene-Tox Program, Mutat Res 1990; 239: 29-80 https://doi.org/10.1016/0165-1110(90)90030-F
  20. Meselson M and Russell K. Comparison of carcinogenesis and mutagenesis potency, Origin of Human Cancer edited by Hiatt HH, Watson JD and Winstend JA. Cold Spring Harbor Laboratory New York, 1991; pp. 1473-1481
  21. Miller BM, Zitzelsberger HF, Weier HUl G and Adler ID. Classification of micronuclei in murine erythrocytes: Immunofluorescent staining using CREST antibodies compared to in situ hybridization with biotinylated gamma satellite DNA, Mutagenesis 1991; 6: 297-302 https://doi.org/10.1093/mutage/6.4.297
  22. Ministry of Labor, Japan, Mutagenicity Test Data of Exist. Chem. Subst., JETOC (Ed..), pp. 384 (1996)
  23. Ministry of Labor, Japan, Mutagenicity Test Data of Exist. Chem. Subst., JETOC (Ed.), (1997) Suppl, pp. 308
  24. Ministry of Labor, Japan, Mutagenicity Test Data of Exist. Chem. Subst., JETOC (Ed.), (1998)
  25. Morita T, Asano N, Awogi T, Sasaki YF, Sato S, Shimada H, Sutou S, Suzuki T, Wakata A, Sofuni T and Hayashi M. Evaluation of the rodent micronucleus assay in the screening of IARC carcinogens (groups 1, 2A and 2B): The summary report of the 6th collaborative study by CSGMT/JEMS.MMS, Mutation Res 1997; 389: 3-122 https://doi.org/10.1016/S1383-5718(96)00070-8
  26. Natl Toxicol Program Tech Rep Ser. 1994; 430: 1-280. NTP Toxicology and Carcinogenesis Studies of C.I. Direct Blue 218 (CAS No. 28407-37-6) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  27. Peace BE and Succop P. Spontaneous micronucleus frequency and age:What are normal values?, Mutat. Res. 1999; 425: 225-230 https://doi.org/10.1016/S0027-5107(99)00030-5
  28. Ryu JC, Lee S, Kim KR and Park J. Evaluation of the genetic toxicity of synthetic chemicals (I). Chromosomal aberration test on chinese hamster lung cells in vitro, Environ. Mutagens & Carcinogens 1994; 14: 138-144
  29. Ryu JC, Kim KR, Ryu EK, Kim HJ, Kwon OS, Song CE, Mar W and Chang IM. Chromosomal aberration assay of taxol and 10-deacetyl baccatin III in chinese hamster lung cells in vitro, Environ. Mutagens & Carcinogens 1996a; 16: 6-12
  30. Ryu JC, Kim KR, Kim HJ, Ryu EK, Lee SY, Jung SO, Youn JY, Kim MH and Kwon OS. Evaluation of the genetic toxicity of synthetic chemicals (II). a pyrethroid insecticide, fenpropathrin, Arch Pharm Res 1996b; 19: 251-257 https://doi.org/10.1007/BF02976235
  31. Ryu JC, Kim KR, Kim HJ, Myung SW, Kim GH, Lee M and Chang IM. Genotoxicity Study of Bojungchisuptang, an oriental herbal decoction-in vitro chromosome aberration assay in chinese hamster lung cells and in vitro supravital-staining micronucleus assay with mouse peripheral reticulocytes, Arch Pharm Res 1998; 21(4): 391-397 https://doi.org/10.1007/BF02974632
  32. Ryu JC, Kim KR and Choi YJ. in vitro mouse lymphoma thymidine kinase $(tk^{+/-})$ gene forward mutation assay in mammalian cells, Environ. Mutagen & Carcinogen 1999; 19(1): 7-13
  33. Ryu JC, Kim KR, Lee S and Park J. Evaluation of the genetic toxicity of synthetic chemicals (III), Chromosomal aberration assay with 28 chemicals in chinese hamster lung cells in vitro, Environ. Mutagens & Carcinogens 2001a; 21(1): 14-22
  34. Ryu JC and Park KY. Anticlastogenic effect of Baechu (Chinese cabbage) Kimchi and Buchu (leek) Kimchi in supravital staining micronucleus assay using peripheral reticulocytes of mouse, Environ Mutagens & Carcinogens 2001b; 21(1): 51-56
  35. Ryu JC, Kim KR and Kim YJ. Evaluation of the Genetic Toxicity of Synthetic Chemicals (IV)-in vitro chromosomal aberration assay with 18 chemicals in Chinese hamster lung cells, Environ. Mutagens & Carcinogens 2002a; 22(3): 149-156
  36. Ryu JC, Kim KR, Kim YJ and Choi HY. Evaluation of the Genetic Toxicity of Synthetic Chemicals (V)-in vitro chromosomal aberration assay with 17 chemicals in Chinese hamster lung cells, Environ. Mutagens & Carcinogens, 2002b; 22(4): 215-222
  37. Ryu JC, Kim KR, Kim YJ and Jeon HK. Evaluation of the genetic toxicity of synthetic chemicals (VI)-in vitro chromosomal aberration assay with 17 chemicals in Chinese hamster lung cells, J Environ Toxicol 2003a; 18(2): 111-120
  38. Ryu JC and Kim KR. Evaluation of the genetic toxicity of synthetic chemicals (VII)-a synthetic selective herbicide, pendimethalin, J Environ Toxicol 2003b; 18(2): 121-129
  39. Ryu JC, Kim KR and Kim YJ. Evaluation of the genetic toxicity of synthetic chemicals (VIII)-in vivo bone marrow micronucleus assay of 8 synthetic chemicals in mice, J Environ Toxicol 2003c; 18(2): 137-143& https://doi.org/10.1002/tox.10111
  40. Ryu JC and Kim YJ. Evaluation of the genetic toxicity of synthetic chemicals (IX)-a synthetic selective herbicide, Pretilachlor, J Environ Toxicol 2004; 19(1): 93-100
  41. Saotome K, Sofuni T and Hayashi M. A micronucleus assay in sea urchin embryos, Mutat Res 1999; 446: 121-127 https://doi.org/10.1016/S1383-5718(99)00155-2
  42. Schmid W. Chemical mutagen testing on in vivo somatic mammalian cells, Agents and Actions 1973; 3: 77-85 https://doi.org/10.1007/BF01986538
  43. Schmid W. The micronucleus test, Mutation Res 1975; 31: 9-15 https://doi.org/10.1016/0165-1161(75)90058-8
  44. Tanisho T, Yagi T, Iwasaki K, Shimoi K, Kinae N, Hayashi M and Sofuni T. Monitoring of coastal water contaminated with mutagens and/ or carcinogens using micronucleus test in fish, Environ. Mutagen Res 1998; 20: 1-9
  45. Tice RR, Erexson GL, Hilliard CJ, Huston JL, Boehm RM, Gulati D and Shelby MD. Effect of treatment protocol and sample time on the frequencies of micronucleated polychromatic erythrocytes in mouse bone marrow and peripheral blood, Mutagenesis 1990; 5(4): 313-21 https://doi.org/10.1093/mutage/5.4.313
  46. U.S. EPA. 1994. Integrated Risk Information System (IRIS) Online. Cincinnati, OH: Office of Health and Environmental Assessment
  47. Westendorf J, Poginsky B, Marquardt H, Groth G, Marquardt H. The genotoxicity of lucidin, a natural component of Rubia tinctorum L., and lucidin ethylether, a component of ethanolic Rubia extracts. Cell Biol Toxicol 1988; 4(2): 225-39 https://doi.org/10.1007/BF00119248