한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제29권3호
  • /
  • Pages.277-285
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  • 2006
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

유산균 발효유 및 starter에서 항생제내성 유산균 분리 및 전달성 R plasmids 분포조사

Isolation of antibiotic resistant Lactobacillus spp from fermented milk and starters, and detection of transmissible R plasmids

  • 백귀정 (전라북도 축산진흥연구소) ;
  • 서형석 (전라북도 축산진흥연구소) ;
  • 노영선 (전라북도 축산진흥연구소) ;
  • 양해동 (전북대학교 수의과대학) ;
  • 허부홍 (전북대학교 수의과대학) ;
  • 서이원 (전라북도 축산진흥연구소) ;
  • 정동석 (전라북도 축산진흥연구소) ;
  • 송희종 (생체안전성연구소 및 응용통계연구소)
  • Baek Kui-Jeong (Jeonbuk Livestock Development & Research Institute) ;
  • Seo Heyng-Seok (Jeonbuk Livestock Development & Research Institute) ;
  • Roh Young-Sun (Jeonbuk Livestock Development & Research Institute) ;
  • Yang Hae-Dong (Statistical Research Institute, Chonbuk National University) ;
  • Hur Boo-Hong (Statistical Research Institute, Chonbuk National University) ;
  • Seo Lee-Won (Jeonbuk Livestock Development & Research Institute) ;
  • Joung Dong-Suk (Jeonbuk Livestock Development & Research Institute) ;
  • Song Hee-Jong (Bio-Safety Research Institute)
  • 발행 : 2006.09.01
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초록

Plasmids are covalently closed circular molecules of DNA that are stably inherited and replicate somewhat independently of the bacterial chromosome. Genes carried on plasmids can mediate a wide variety of important functions, including antibiotics (R plasmids) and heavy metals resistance, toxins production, cell penetration, iron chelation, complement resistance, and metabolic characteristics such as sucrose and lactose fermentation. Fifty strains of lactobacilli were isolated from 26 staters and 29 fermented milk products. They were classified 27 strains as Lactobacillus paracasei subsp paracasei, 11 stains as Lactococcus lactis subsp cremoris, 6 strains as L delbrueckii subsp lactis, 4 strains as L acidophius, and 2 strains as L delbrueckii subsp bulgaricus. All of these strains were examined for drug resistance and transferability of R plasmids. All of the isolates were sensitive to Am, C, CF, E, NB, P, T, and Te. But resistant to SXT 94% (47 strains), K 66% (33 strains), S 56% (28 strains), ENR 50% (25 strains), NOR 38% (19 strains) CIP 38% (19 strains), GM 16% (8 strains), and N 14% (7 strains), in order. And 32 different resistant patterns were found. The most frequently encountered patterns were CIP-ENR-K-NOR-S-SXT (5 strains). In vitro R plasmids transfer experiment, 57 antibiotic resistant strains which were not transfer to the recipient 2 Escherichia coli strains by conjugation, These results indicate that Lactobacillus in internal trade market' stater recognize R factor but transmissible R plasmid is not existed.

키워드

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