Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effects of Oral Administration of Thyroid Hormone on Physiological Activity and Growth of Black Porgy Reared in Freshwater or Seawater

담수 및 해수사육 감성돔, Acanthopagrus schlegeli의 생리활성과 성장에 미치는 갑상선 호르몬의 효과

  • Min Byung-Hwa (Department of Aquaculture, Pukyong National University) ;
  • Noh Gyoung-Ane (Department of Aquaculture, Pukyong National University) ;
  • Jeong Min-Hwan (Department of Aquaculture, Pukyong National University) ;
  • Kang Duk-Young (West Sea Fisheries Research Institute, NFRDI) ;
  • Choi Cheol-Young (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Bang In-Chul (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Chang Young-Jin (Department of Aquaculture, Pukyong National University)
  • 민병화 (부경대학교 수산과학대학 양식학과) ;
  • 노경언 (부경대학교 수산과학대학 양식학과) ;
  • 정민환 (부경대학교 수산과학대학 양식학과) ;
  • 강덕영 (국립수산과학원 서해수산연구소) ;
  • 최철영 (한국해양대학교 해양.환경생명과학부) ;
  • 방인철 (순천향대학교 해양생명공학과) ;
  • 장영진 (부경대학교 수산과학대학 양식학과)
  • Published : 2006.08.01
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Abstract

Stress responses, osmoregulation and growth of black porgy reared in freshwater or seawater were investigated by supplying the feed (10 mg/kg feed) treated with the $T_3$ (3,5,3'-triiodo-L-thyronine) for 90 days. Plasma $T_3$ in black porgy reared for 60 days in freshwater with (FT) or without (FN) $T_3$, treatment was lower than that in black porgy reared in seawater with (ST) or without (SN) $T_3$ treatment. The concentration of $T_3$ in FT was significantly more than that in FN. Plasma cortisol in FT, at the same day, was 4 times higher than that in FN, whereas the differences of the hormone between ST and SN was not significant. Although the levels of plasma glucose in FN and FT tended to be lower than that in ST and SN, no differences were found between fish reared in freshwater and seawater after $T_3$ treatment. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of black porgy reared in seawater showed no difference throughout the experimental period. However, at the beginning stages of the experiment, the plasma levels of these enzymes in FN and FT were higher than in SN and ST. Recovering of AST level to the level of SN or FT was laster in FT than in FN. However, no effects of the exogenous $T_3$ treatment on osmoregulatory capacity and growth of black porgy reared in freshwater or seawater were observed in this study.

본 연구에서는 담수 및 해수사육 감성돔을 대상으로 $T_3$가 처리된 사료(10 mg/kg사료)를 90일간 공급하면서 어체의 스트레스, 삼투압조절 및 성장을 조사하였다. 혈장 $T_3$ 농도는 60일째에 FN, FT이 SN, ST에 비해 낮았으며, 또한 FN은 FT보다 유의하게 낮았다. 혈장 cortisol은 60일째를 제외하고는 실험구 사이에서 차이가 없었다 60일째에 FT $(21.0{\pm}3.3ng/ml)$는 $FN(5.1{\pm}1.4ng/ml)$보다 4배정도 높았으며, 또한 유의한 차이는 인정되지 않았지만 ST가 SN에 비해 다소 높은 것으로 나타났다. FN, FT의 혈장 glucose는 실험기간동안 SN, ST에 비해 낮은 경향을 보였으나, 담수 및 해수사육 감성돔에서 호르몬 처리에 의한 차이는 나타나지 않았다. 해수사육 감성돔의 AST 및 ALT는 실험기간동안 차이가 없었으나, 실험개시시 FN, FT의 AST및 ALT는 SN, ST보다 높았다. 또한 FN, FT의 AST의 해수수준으로 회복은 각각 실험종료시와 30일째에 해수수준으로 나타나 호르몬을 처리한 FT가 더 빨랐다. 그러나, 담수 및 해수사육 감성돔의 삼투압조절 향상 및 성장촉진을 위한 외인성 $T_3$ 공급 효과는 나타나지 않았다.

Keywords

References

  1. Brown, C. L. and H. A. Bern, 1989. Hormones in early development with special reference to teleost fishes. (In) Hormones in Development, Maturation and Senescence of Neuroendocrine Systems, Academic Press, New York, pp. 289-306
  2. Chang, Y. J., B. H. Min, H. J. Chang and J. W. Hur, 2002. Comparison of blood physiology in black seabream (Acanthopagrus schlegeli) cultured in converted freshwater from seawater and seawater from freshwater. J. Korean Fish. Soc., 35, 595-600
  3. Denver, R. J., 1998. The molecular basis of thyroid hormonedependent central nervous system remodeling during amphibian metamorphosis. Comp. Biochem. Physiol., 119, 219-228
  4. Dickhoff, W. W., L. Yan, E. M. Plisetskaya, C. V. Sullivan, P. Swanson, A. Hara and M. G. Bernard, 1989. Relationship between metabolic and reproductive hormones in salmonid fish. Fish Physiol. Biochem., 7, 147-155 https://doi.org/10.1007/BF00004701
  5. Foo, J. T. W. and T. J. Lam, 1993a. Retardation of ovarian growth and depression of serum steroid levels in tilapia, Oreochromis mossambicus by cortisol implantation. Aquaculture, 115, 133- 143 https://doi.org/10.1016/0044-8486(93)90364-5
  6. Foo, J. T. W. and T. J. Lam, 1993b. Serum cortisol response to handling stress and the efeect of cortisol implantation on testosterone level in the tilapia, Oreochromis mossambicus. Aquaculture, 115, 145-158 https://doi.org/10.1016/0044-8486(93)90365-6
  7. Goswami, S. V. and B. I. Sundararaj, 1974. Effects of $C_{18},\;C_{19},\; and\;C_{21}$ steroids on in vitro maturation of oocytes of the catfish, Heteropneustes fossilis (Bloch). Gen. Comp. Endocrinol., 23, 282-285 https://doi.org/10.1016/0016-6480(74)90071-9
  8. Higgs, D. A., B. S. Dosanjh, L. M. Uin, B. A. Himick and J. G. Eales, 1992. Effects of dietary lipid and carbohydrate levels and chronic 3,5,3'-triiodo-L-thyronine treatment on growth, appetite, food and protein utilization and body composition of immature rainbow trout, Oncorhynchus mykiss, at low temperature. Aquaculture, 105, 175-190 https://doi.org/10.1016/0044-8486(92)90129-9
  9. Higgs, D. A., U. H. M. Fagerlund, J.R. McBride and J. G. Eales, 1979. Influence of orally administered L-thyroxine or 3,5,3'-triiodo- L-thyronine on growth, food consumption and food conversion of underyearling coho salmon (Oncorhynchus kisutch). Can. J. Zool., 57, 1974-1979 https://doi.org/10.1139/z79-261
  10. Kang, D. Y. and Y. J. Chang, 1996. Effects of dietary 3,5,3'-triiodo- L-thyronine ($T_3$) on growth and survival rate in juvenile black seabream, Acanthopagrus schlegeli. J. Aquaculture, 9, 215-222
  11. Kang, D. Y., Y. J. Chang, Y. Kim and J. I. Myoung, 2001. Effects of oral administrated thyroid hormone ($T_3$) on physiological condition, growth and survival rate of juvenile rockfish (sebastes schlegeli). J. Korean Fish. Soc. 34, 588-593
  12. Kime, D. E., 1998. Disruption of thyroid and interrenal function. (In) Endocrine Disruption in Fish. Kluwer Academic Publishers, MA, USA, pp. 247-274
  13. Kitajima, C. and Y. Tsukashima, 1983. Morphology, growth and low temperature and low salinity tolerance of sparid hybrids (Sparus sarba, Acanthopagrus schlegeli). Jap. J. Ichthyol., 30, 275-283
  14. Lam, T. J., 1980. Thyroxine enhances larval development and survival in Sarotherodon (Tilapia) mossambicus. Aquaculture, 21, 287-291 https://doi.org/10.1016/0044-8486(80)90138-6
  15. Leatherland, J. F. and S. B. Barrett, 1993. Investigation into the development of the pituitary gland-thyroid tissue axis and distribution of tissue thyroid hormone content in embryonic coho salmon (Oncorhynchus kisutch) from Lake Ontario. Fish Physiol. Biochem., 12, 149-159 https://doi.org/10.1007/BF00004380
  16. Lee, Y. H., J. L. Dub, Y. S. Shih, S. R. Jeng, L. T. Sun and C. F. Chang, 2004. In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, Acanthopagrus schlegeli. Gen. Comp. Endocrinol., 139, 12-19 https://doi.org/10.1016/j.ygcen.2004.07.010
  17. Madsen, S. S. and B. Korsgaard, 1989. Time-course effects of repetitive estradiol-17$\beta$ and thyroxine injections on the natural spring smolting of Atlantic salmon, Salmo salar L. J. Fish Biol., 35, 119-128 https://doi.org/10.1111/j.1095-8649.1989.tb03398.x
  18. Mancera, J. M. and S. D. McCormick, 1999. Influence of cortisol, growth hormone, insulin-like growth factor I and 3,3',5-triiodol- thyronine on hypoosmoregulatory ability in the euryhaline teleost Fundulus heteroclitus. Fish Physiol. Biochem., 21, 25-33 https://doi.org/10.1023/A:1007737924339
  19. Min, B. H., B. K. Kim, J. W. Hur, I. C. Bang, S. K. Byun, C. Y. Choi and Y. J. Chang, 2003. Physiological responses during freshwater acclimation of seawater-cultured black porgy (Acanthopagrus schlegeli). Korean J. Ichthyol., 15, 224-231
  20. Min, B. H., C. Y. Choi and Y. J. Chang, 2005a. Comparison of physiological conditions on black porgy, Acanthopagrus schlegeli acclimated and reared in freshwater and seawater. J. Aquaculture, 18, 37-44
  21. Min, B. H., G. A. Noh, M. H. Joeng and Y. J. Chang, 2005b. Effects of 3,5,3'-triiodo-L-thyronine ($T_3$) on sex steroid levels and gonadal development in black porgy, Acanthopagrus schlegeli. Dev. Reprod., 9, 15-22
  22. Nacario, J. F., 1983. The effect of thyroxine on the larvae and fry of Sarotherodon niloticus L. (Tilapia nilotica). Aquaculture, 34, 73-83 https://doi.org/10.1016/0044-8486(83)90292-2
  23. Pan, C. H., Y. H. Chien and B. Hunter, 2003. The resistance to ammonia stress of Penaeus monodon Fabricius juvenile fed diets supplemented with astaxanthin. J. Exp. Mar. Biol. Ecol., 297, 107-118 https://doi.org/10.1016/j.jembe.2003.07.002
  24. Peter, M. C. S., R. A. C. Lock and S. E. Wendelaar Bonga, 2000. Evidence for an osmoregulatory role of thyroid hormones in the freshwater mozambique tilapia Oreochromis mossambicus. Gen. Comp. Endocrinol., 120, 157-167 https://doi.org/10.1006/gcen.2000.7542
  25. Saunders, R. L., S. D. McCormick, E. B. Henderson, J. G. Eales and C. E. Johnston, 1985. The effect of orally administered 3,5,3'-triiodo-L-thyronine on growth and salinity tolerance of Atlantic salmon (Salmo salar L.). Aquaculture, 45, 143-156 https://doi.org/10.1016/0044-8486(85)90265-0
  26. Schreiber, A. and J. Specker, 1999. Early larval development and metamorphosis in the summer flounder: changes in percent whole-body water content and effects of altered thyroid status. J. Fish Biol., 55, 148-157 https://doi.org/10.1111/j.1095-8649.1999.tb00664.x
  27. Shi, Y. B. and D. D. Brown, 1993. The earliest changes in tadpole intestine induced by thyroid hormone. J. Biol. Chem., 268, 20312-20317
  28. Szischa, V., N. Papandroulakis and M. Pavlidis, 2005. Ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, Sparus aurata. Gen. Comp. Endocrinol., 142, 186-192 https://doi.org/10.1016/j.ygcen.2004.12.013
  29. Tanaka, M., J. B. Tanangonan, M. Tagawa, E.G. de Jesus, H. Nishida, M. Isaka, R, Kimura and T. Hirano, 1995. Develop ment of the pituitary, thyroid and interrenal glands and application of endocrinology to the improved rearing of marine fish larvae. Aquaculture, 135, 111-126 https://doi.org/10.1016/0044-8486(95)01019-X
  30. Wendelaar Bonga, S. E., 1993. Endocrinology. In 'The Physiology of Fishes' (D. H. Evans, Ed.), CRC Press, Boca Raton, FL., pp. 469-502
  31. 池田彌生, 尾崎久雄, 瀨崎哲次郞. 1986. 魚類血液圖鑑. 綠書房, 東京, pp. 361