Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Production of Egg Yolk Immunoglobulin and Its Application for Pepper mild mottle virus in Serological Tests

Pepper mild mottle virus에 대한 난황항체의 생산과 혈청학적 진단에의 활용

  • Han Jung-Heon (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee Cheol-Ho (Department of Biological Engineering, College of Natural Sciences and Technology, Seokyoung University) ;
  • Kim Young-Ho (Department of Agricultural Biotechnology, Seoul National University) ;
  • La Yong-Joon (Department of Agricultural Biotechnology, Seoul National University)
  • 한정헌 (서울대학교 농생명공학부) ;
  • 이철호 (서경대학교 이공대학 생물공학과) ;
  • 김영호 (서울대학교 농생명공학부) ;
  • 나용준 (서울대학교 농생명공학부)
  • Published : 2006.08.01
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Abstract

Egg yolk immunoglobulin (IgY) is much widely used in medical fields, but its use in serology of plant viruses is much limited. We produced an IgY against pepper mild mottle virus (PMMoV) and applied it to several serological tests. Polyclonal antibodies were obtained from the egg yolk of chicken immunized with a total of 2mg of purified PMMoV over 2 months. The titers of antibodies were measured with the ring-test over six months after the first injection. The highest.titers of IgY was 1/2,560 at 2 months after the first injection. Approximately 60-80 mg of IgY were obtained from one egg yolk. Using the IgY, 1ng/ml of purified PMMoV was detected with the indirect ELISA. Gelrite gel double diffusion test, ELISA and tissue immuno-binding assay employing IgY gave similar sensitivity and specificity to those of IgG developed in rabbit. Therefore, the IgY which can be obtained in large quantities from a chicken, might be useful for the antibody production and the serology of plant viruses.

닭을 이용한 egg yolk immunoglobulin (IgY)의 생산과 이용 가능성을 조사하기 위하여 pepper mild mottle virus (PMMoV)로 닭을 면역하였다. Ring-test로 달걀의 난황에서 분리 정제한 IgY의 역가를 조사한 결과 IgY의 최고 역가는 1:2,560이었고 달걀 l 개에서 대략 $60{\sim}80 mg$의 IgY를 얻을 수 있었다. 최고역가를 갖는 IgY를 이용하여 Gelrite gel 이중확산법, 효소면역항체법 (ELISA), 조직자국면역반응법을 실시한 결과 간접면역효소항체법의 PMMoV 검출 감도는 1 ng/ml 이었고, 검정법의 바이러스 검출감도와 특이도는 IgG와 비슷한 경향을 보였다. 그러므로 한 마리의 닭으로부터 대량으로 생산 가능한 IgY는 식물바이러스에 대한 항체생산과 혈청학적 검정에 유용할 것으로 생각한다.

Keywords

References

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