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http://dx.doi.org/10.5423/RPD.2006.12.2.144

Production of Egg Yolk Immunoglobulin and Its Application for Pepper mild mottle virus in Serological Tests  

Han Jung-Heon (Department of Agricultural Biotechnology, Seoul National University)
Lee Cheol-Ho (Department of Biological Engineering, College of Natural Sciences and Technology, Seokyoung University)
Kim Young-Ho (Department of Agricultural Biotechnology, Seoul National University)
La Yong-Joon (Department of Agricultural Biotechnology, Seoul National University)
Publication Information
Research in Plant Disease / v.12, no.2, 2006 , pp. 144-147 More about this Journal
Abstract
Egg yolk immunoglobulin (IgY) is much widely used in medical fields, but its use in serology of plant viruses is much limited. We produced an IgY against pepper mild mottle virus (PMMoV) and applied it to several serological tests. Polyclonal antibodies were obtained from the egg yolk of chicken immunized with a total of 2mg of purified PMMoV over 2 months. The titers of antibodies were measured with the ring-test over six months after the first injection. The highest.titers of IgY was 1/2,560 at 2 months after the first injection. Approximately 60-80 mg of IgY were obtained from one egg yolk. Using the IgY, 1ng/ml of purified PMMoV was detected with the indirect ELISA. Gelrite gel double diffusion test, ELISA and tissue immuno-binding assay employing IgY gave similar sensitivity and specificity to those of IgG developed in rabbit. Therefore, the IgY which can be obtained in large quantities from a chicken, might be useful for the antibody production and the serology of plant viruses.
Keywords
Egg yolk immunoglobulin (IgY); Antibody; Pepper; Pepper mild mottle virus (PMMoV);
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