Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of a Noncanonical Purine dNTP Pyrophosphatase from Archaeoglobus fulgidus

  • Im Eun-Kyoung (Yonsei Research Institute of Aging Science, Yonsei University) ;
  • Hong Chang-Hyung (Yonsei Research Institute of Aging Science, Yonsei University) ;
  • Back Jung-Ho (Department of Advanced Fusion Technology, Konkuk University) ;
  • Han Ye-Sun (Department of Advanced Fusion Technology, Konkuk University) ;
  • Chung Ji-Hyung (Yonsei Research Institute of Aging Science, Yonsei University)
  • Published : 2006.07.01
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Abstract

DNA can oxidatively be deaminated by ROS, which converts DNA base amino groups to keto groups and can trigger abnormal mutations, resulting in mutagenesis in organisms. In this study, a noncanonical purine dNTP pyrophosphatase (AfPPase) from a hyperthermophilic archaeon Archaeoglobus fulgidus, which hydrolyzes aberrant nucleoside triphosphates, was overexpressed in E. coli, purified, and characterized. The purified AfPPase showed remarkably high activity for XTP and dITP, suggesting that the 6-keto group of these nucleotides is critical for the reactivity. Under optimal reaction conditions, the reaction rate for these substrates was about 120 times that with dGTP. Therefore, AfPPase may play a significant role in DNA repair by hydrolysis of noncanonical nucleotides before they are misincorporated into DNA.

Keywords

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