Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Optimal Culture Conditions and Isolation of a ι-Carrageenase-producing Marine Bacterium

  • Shim Hang-Sun (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Lim Su-Jin (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Choi Min-Jung (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Kim Jong-Oh (EnbioGene Co. Ltd., Department of Food and Life Science, Pukyong National University) ;
  • Kim Seok-Ryel (EnbioGene Co. Ltd., Department of Food and Life Science, Pukyong National University) ;
  • Kim Hyeung-Rak (Division of Food Science and Biotechnology, Pukyong National University)
  • Published : 2006.06.01
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Abstract

A bacterial strain capable of hydrolyzing carrageenan was isolated from the coast of Busan in Korea. The isolated strain (HS5322) is aerobic, gram-negative, rod-shaped, and motile. Comparison of the 16S rDNA of the isolate with that of known Pseudomonas sp. showed that sequence similarity was at most 95%, implying that the isolate is a new Pseudomonas species. The organism grew optimally at NaCl concentrations of 2.0 to 2.5%. The optimum temperature and pH for carrageenase production in a 72-h flask culture containing 1% carrageenan was $20^{\circ}C$ and pH 8.5, respectively. Of the synthetic substrates tested, the highest enzyme activity was obtained with p-nitrophenyl ${\beta}$-D-galactopyranoside.

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References

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