Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Role of CaBud6p in the Polarized Growth of Candida albicans

  • Song Yun-Kyoung (School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim Jeong-Yoon (School of Bioscience and Biotechnology, Chungnam National University)
  • Published : 2006.06.01
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Abstract

Bud6p is a component of a polarisome that controls cell polarity in Saccharomyces cerevisiae. In this study, we investigated the role of the Candide albicans Bud6 protein (CaBud6p) in cell polarity and hyphal development. CaBud6p, which consists of 703 amino acids, had 37% amino-acid sequence identity with the Bud6 protein of S. cerevisiae. The homozygous knock-out of CaBUD6 resulted in several abnormal phenotypes, such as a round and enlarged cells, widened bud necks, and a random budding pattern. In hypha-inducing media, the mutant cells had markedly swollen tips and a reduced ability to switch from yeast to hypha. In addition, a yeast two-Hybrid analysis showed a physical interaction between CaBud6p and CaAct1p, which suggests that CaBud6p may be involved in actin cable organization, like Bud6p in S. cerevisiae. Taken together, these results indicate that CaBud6 plays an important role in the polarized growth of C. albicans.

Keywords

References

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