Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhancement of Astaxanthin Production of Haematococcus pluvialis by Mutation

돌연변이를 통한 미세조류 Haematococcus pluvialis의 Astaxanthin 생산성의 향상

  • Park Bok-Jun (Institute of Industrial Biotechnology, Department of Biological Engineering Inha University) ;
  • Kim Beob-Min (Institute of Industrial Biotechnology, Department of Biological Engineering Inha University) ;
  • Shim Su-Hyun (Institute of Industrial Biotechnology, Department of Biological Engineering Inha University) ;
  • Kim Jeong-Dong (Institute of Industrial Biotechnology, Department of Biological Engineering Inha University) ;
  • Lee Choul-Gyun (Institute of Industrial Biotechnology, Department of Biological Engineering Inha University)
  • 박복준 (인하대학교 공과대학 생물공학과/생물산업기술연구소) ;
  • 김법민 (인하대학교 공과대학 생물공학과/생물산업기술연구소) ;
  • 심수현 (인하대학교 공과대학 생물공학과/생물산업기술연구소) ;
  • 김정동 (인하대학교 공과대학 생물공학과/생물산업기술연구소) ;
  • 이철균 (인하대학교 공과대학 생물공학과/생물산업기술연구소)
  • Published : 2006.06.01
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Abstract

Haematococcus pluvialis is a great producer of astaxanthin (3,3'-dihydroxy-$\beta$,$\beta$-carotene-4,4'-dione). The activities of astaxanthin include potential cancer prevention, immune response enhancement, antioxidant activity, and so on. Nevertheless, it tried to manipulate by mutation for overcoming low growth rate of wild type and limited production of astaxanthin. Mutated colony that is lager and more reddish one than wild type was selected by attempting to expose strains to UV irradiation and to treat chemical such as EMS and colchicines as mutagen. Selected mutants were further screened using inhibitors of the carotenoid biosynthetic pathway. Inhibitors used were nicotine and diphenylamine and both had decreased the survival rate by 40-50%. Among over 50,000 mutant colonies screened, two strains were selected. One selected mutant strain (U15-5) from UV treatment showed 1.68-fold higher total carotenoid contents per cell than that of the wild type strain. On the other hand, the other selected mutant strains (DS, M4-3) from colchicine treatment showed 20$\sim$30% faster cell growth than the wild type strain.

Haematococcus pluvialis는 astaxanthin을 많이 생산하는 미세조류로써 astaxanthin은 항산화제로 면역반응을 강화시켜주며 항암효과 등을 가지고 있다. 그런데 야생종의 낮은 생장속도와 astaxanthin의 생산에는 한계가 있기 때문에 이를 극복하기위해 돌연변이 방법을 사용하였다. 돌연변이 방법으로는 자외선 조사와 EMS와 colchicines 처리를 사용하여 야생종보다 colony가 크고 더 붉은 것은 선택하였다. 선별된 돌연변이들은 carotenoid 생합성과정을 억제하는 nicotine 과 diphenylamine을 이용하여 다시 선별하였다. 그때 생존율은 40-50%이었고 여기서 선별된 균주들을 다시 규모를 키워 배양한 결과 자외선 처리한 돌연변이인 U15-5가 야생종보다 세포당 total carotenold 생산량이 1.68배 증가하였고, colchicine 처리한 DS와 M4-3은 생장속도가 $20\sim30%$ 증가하였다.

Keywords

References

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