Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Taxonomy of a Soil Bacteria YNB54 Strain Which Shows Specific Antagonistic Activities against Plant Pathogenic Phytophthora spp.

식물역병균 Phytophthora spp.에 특이 길항균인 YNB54 균주의 분류

  • Kim Sam-Sun (Microbial Genetics Division, National Institute of Agricultural Biotechnology) ;
  • Kwon Soon-Wo (Microbial Genetics Division, National Institute of Agricultural Biotechnology) ;
  • Lee Seon-Young (Microbial Genetics Division, National Institute of Agricultural Biotechnology) ;
  • Kim Soo-Jin (Microbial Genetics Division, National Institute of Agricultural Biotechnology) ;
  • Koo Bon-Sung (Microbial Genetics Division, National Institute of Agricultural Biotechnology) ;
  • Weon Hang-Yeon (Applied Microbiology Division, National Institute of Agricultural Science) ;
  • Kim Byung-Yong (Microbial Genetics Division, National Institute of Agricultural Biotechnology) ;
  • Yeo Yun-Soo (Microbial Genetics Division, National Institute of Agricultural Biotechnology) ;
  • Lim Yoong-Ho (Department of Applied Biology Chemistry, Konkuk University) ;
  • Yoon Sang-Hong (Microbial Genetics Division, National Institute of Agricultural Biotechnology)
  • 김삼선 (농업생명공학원 미생물유전과) ;
  • 권순우 (농업생명공학원 미생물유전과) ;
  • 이선영 (농업생명공학원 미생물유전과) ;
  • 김수진 (농업생명공학원 미생물유전과) ;
  • 구본성 (농업생명공학원 미생물유전과) ;
  • 원항연 (농업과학기술원 응용미생물과) ;
  • 김병용 (농업생명공학원 미생물유전과) ;
  • 여윤수 (농업생명공학원 미생물유전과) ;
  • 임융호 (건국대학교 응용생물화학과) ;
  • 윤상홍 (농업생명공학원 미생물유전과)
  • Published : 2006.06.01
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Abstract

YNB54 strain which shows inhibitory activities specific to the plant pathogenic Phytophthora sp. on potato dextrose agar medium was screened among lots of strains isolated from Korean soils. To identify taxonomy of the Phytophthora specific antagonistic bacteria YNB54, 165 rDNA sequence, MIDI fatty acid composition, DNA-DNA hybridization, GC content, and commercial multitest systems such as API 20E and Biolog GN were performed. Results of commercial kits including lots of biochemical and physiological reactions showed that this strain was closely related to taxa including Enterobacter cloacae and Enterobacter cancerogenus species than other genera(Citerobacter Klebsiella, Leclercia). Also, analysis of its MIDI, G+C contents, and DNA-DNA hybridization suggests that this strain was more similiar to the Genus Enterobacter than other genera (Citerobacter Klebsiella, Leclercia). This strain was potentially identified as Enterobacter sp. by these results. But our 16S ribosomal DNA sequences (rDNA) analysis confirmed that it was more closely related to the cluster of Citerobacter freundii ATCC 29935 than any other Enterobacter species. In the absence of defined phylogenetic critia for delineating genera, the results observed with Citrobacter and Enterobacter species suggest that further studies are needed to clarify their relationships. This investigation demonstrates that YNB54 strain is genetically diverse and potentially more taxonomically complex than hitherto realized. Further study is necessary to confirm their taxonomic positions.

Phytophthora sp.의 균사성장을 특이적으로 저해하는 토양 미생물인 YNB54 균주의 정확한 분류적 위치를 밝히기 위해 Biolog GN2, API 20E와 같은 상업적 생화학 kit, 16S rDNA, DAN-DNA hybridization, GC함량, MIDI 등의 분석을 수행하였다. 다양한 생화학적 kit를 사용한 동정 결과는 이 균주가 다른 어떤 종보다 Enterobacter cloacae와 E. cancerogenus에 보다 더 가까움을 보여주었다. 또한 DAN-DNA hybridization, GC함량, MIDI 분석의 결과들 역시 다른 속 (Citerobacter, Klebsiella, Leclercia)보다 Enterobacter 속에 더 유사함을 암시해 주었다. 그러나 16S rDNA분석에서 이 균주는 Citrobacter freundii(99.4%)와 동일 그룹으로 구분되었지만 Enterobacter, Leclecia, Klebsiella 속 등과도 98%이상의 상동성을 보여주는 polyphyletic 특성을 보였다. 결론적으로 YNB54의 분류 동정을 위한 우리의 조사들은 이 균주가 유전적으로 다양하고 지금까지 아는 것보다 분류학적으로 더 복잡함을 암시해줌에도 불구하고 Enterobacter속임이 가장 유력하다는 것을 보여 주었다.

Keywords

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