Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Proteins Affected by Iron in Saccharomyces cerevisiae Using Proteome Analysis

  • Lieu Hae-Youn (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Song Hyung-Seok (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Yang Seung-Nam (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim Jae-Hwan (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim Hyun-Joong (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Park Young-Doo (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Park Cheon-Seok (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim Hae-Yeong (Institute of Life and Resources and Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2006.06.01
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Abstract

To study the effect of iron on Saccharomyces cerevisiae, whole-cell proteins of Saccharomyces cerevisiae were extracted and subjected to two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and differentially expressed proteins were identified. The proteins separated were further identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and were compared with a protein database. Of more than 300 spots separated by molecular weight and isoelectric points, 27 differentially expressed spots were identified. Ten proteins were found to be differentially expressed at high iron concentration. Triosephosphate isomerase (TPI), YDR533C hypothetical protein, superoxide dismutase (SOD), 60 kDa heat-shock protein (HSP60), pyruvate dehydrogenase beta subunit 1 (PDB1), and old yellow enzyme 2 (OYE2) were upregulated, whereas thiol-specific antioxidant (TSA), regulatory particle non-ATPase subunit 8 (RPN8), thiol-specific peroxiredoxin 1 (AHP1), and fructose-1, 6-bisphosphate adolase (FBA) were downregulated by iron. Based on the result, we propose that SOD upregulated by iron would protect the yeast from oxidative stress by iron, and that TSA downregulated by iron would render cells hypersensitive to oxidative stress.

Keywords

References

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