Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Influence of Level of Feed Intake on Concentration of Purine Derivatives in Urinary Spot Samples and Microbial Nitrogen Supply in Crossbred Bulls

  • George, S.K. (Animal Nutrition Division, Indian Veterinary Research Institute) ;
  • Dipu, M.T. (Animal Nutrition Division, Indian Veterinary Research Institute) ;
  • Mehra, U.R. (Animal Nutrition Division, Indian Veterinary Research Institute) ;
  • Verma, A.K. (Animal Nutrition Division, Indian Veterinary Research Institute) ;
  • Singh, P. (Animal Nutrition Division, Indian Veterinary Research Institute)
  • Received : 2005.04.11
  • Accepted : 2005.11.11
  • Published : 2006.09.01
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Abstract

The potential of the spot urine sampling technique as an alternative to performing a total urine collection to predict the microbial nitrogen supply was evaluated in crossbred bulls. In a completely randomized design, 20 growing crossbred bulls were assigned four levels of feed intake (120, 100, 80 and 60% of voluntary dry matter intake) on diets comprised of wheat straw and concentrate mixture (50:50). After three months of experimental feeding, a metabolism trial was conducted for ten days, during which spot urine collections were performed every 6 h post feeding on days 9 and 10. The daily urinary excretion of allantoin (A) and purine derivatives (PD) decreased with the reduction in feed intake while creatinine (C) excretion remained similar in animals fed at different levels. The microbial nitrogen (MN) supply calculated from the PD excreted in total urine (35.08 to 72.08 g/d) was higher at increased levels of feed intake. PD concentration in spot urine samples had poor correlation with feed intake except at 12 h post feeding. A/C ratio and PD/C ratio in spot urine samples remained similar irrespective of sampling time and significantly (p<0.01) correlated with daily urinary PD excretion, digestible organic matter intake and dry matter (DM) intake. However, no significant differences were evident in these ratios among animals fed at levels 120, 100 and 80% of voluntary dry matter intake (VDMI) at different times post feeding. These results suggests that the spot urine sampling technique to predict the microbial protein supply is not suitable for detecting small differences in MN supply and hence, estimation of PD excreted in total urine (mmol/d) is necessary to assess precisely the MN supply in crossbred bulls.

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References

  1. Agricultural Research Council. 1984. The nutrient requirements of ruminant livestock. Supplement 1. Commonwealth Agricultural Bureaux, Farningham Royal, Sough
  2. Antoniewicz, A. M., W. W. Heinemann and E. M. Hanks. 1980. The effect of changes in the intestinal flow of nucleic acids on allantoin excretion in the urine of sheep. J. Agric. Sci. (Camb.). 95:395-400 https://doi.org/10.1017/S0021859600039435
  3. AOAC. 1984. Official methods of analyses, 14th ed. Association of Official Analytical Chemists. Washington, DC
  4. AOAC. 1995. Official methods of analyses, 16th ed. Association of Official Analytical Chemists. Washington, DC
  5. Balcells, J., D. S. Parker and C. J. Seal. 1992b. Purine metabolite concentrations in portal and peripheral blood of steers, sheep and rats. Comp. Biochem. Physiol. 101B:633-636
  6. Balcells, J., J. A. Guada, J. M. Peiro and D. S. Parker. 1992a. Simultaneous determination of allantoin and oxypurines in biological fluids by high performance liquid chromatography. J. Chromatography 575:153-157 https://doi.org/10.1016/0378-4347(92)80517-T
  7. Blummel, M., E. Zerbini, B. V. S. Reddy, C. T. Hash, F. Bidinger and D. Ravi. 2003. Improving the production and utilization of sorghum and pearl millet as livestock feed: methodological problems and solutions. Field Crops Res. 84:123-142 https://doi.org/10.1016/S0378-4290(03)00145-X
  8. Chanjula, P., M. Wanapat, C. Wachirapakorn and P. Rowlinson. 2004. Effect of various levels of cassava hay on rumen ecology and digestibility in Swamp Buffaloes. Asian-Aust. J. Anim. Sci. 17:663-669 https://doi.org/10.5713/ajas.2004.663
  9. Chen, X. B. and M. J. Gomes. 1992. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives. An overview of the technical details. Occassional Publication, International Feed Resources Unit. The Rowett Research Institute, University of Aberdeen, UK
  10. Chen, X. B., F. D. DeB. Hovell, E. R. Orskov and D. S. Brown. 1990. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivatives excretion in sheep. Br. J. Nutr. 63:131-142 https://doi.org/10.1079/BJN19900098
  11. Chen, X. B., G. Grubic, E. R. Orskov and P. Osuji. 1992. Effect of feeding frequency on diurnal variation in plasma and urinary purine derivatives in steers. Anim. Prod. 55:185-191 https://doi.org/10.1017/S0003356100037442
  12. Chen, X. B., P. Susmel, B. Stefanson and E. R. Orskov. 1995. Evaluation of the use of the purine derivative: creatinine ratio in the spot urine and plasma samples as an index of microbial protein supply in sheep and cattle. In: Protein Metabolism and Nutrition (Ed. A. F. Numes, A. V. Portugal, J. P. Costa and J. R Ribiero) Estacao Zootecnica National, Santarem, Portugal, pp. 325-329
  13. Daniels, Z. M., X. B. Chen, D. J. Kyle, K. Sinclair and E. R. Orskov. 1994. Purine derivatives in urine and plasma of lactating cows given different levels of food intake. Anim. Prod. 58:483 (Abstr.)
  14. Dehority, B. A. and C. G. Orpin. 1988. Development of natural fluctuations in rumen microbial populations. In The Rumen Microbial Ecosystem (Ed. P. N. Hobson). Elsevier, London, pp. 151
  15. Dipu, M. T., S. K. George, P. Singh, A. K. Verma and U. R. Mehra. 2006. Measurement of microbial protein supply in Murrah buffaloes (Bubalus bubalis) using urinary purine derivatives excretion and PDC index. Asian-Aust. J. Anim. Sci. 19:347-355 https://doi.org/10.5713/ajas.2006.347
  16. Djouvinov, D. S. and N. A. Todorov. 1994. Influence of dry matter intake and passage rate on microbial protein synthesis in the rumen of sheep and its estimation by cannulation and a noninvasive method. Anim. Feed Sci. Technol. 48:289-304 https://doi.org/10.1016/0377-8401(94)90179-1
  17. Dynes, R. A., D. A. Henry and D. G. Masters. 2003. Characterizing forages for ruminant feeding. Asian-Aust. J. Anim. Sci. 16:116-123 https://doi.org/10.5713/ajas.2003.116
  18. Fujihara, T., E. R. Orskov, P. J. Reed and D. J. Kyle. 1987. The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. J. Agri. Sci. (Camb.) 109:7-12 https://doi.org/10.1017/S0021859600080916
  19. Galyean, M. L. and F. N. Owens. 1991. Effects of diet composition and level of feed intake on site and extent of digestion in ruminants. In Physiological Aspect of Digestion and Metabolism in Ruminants (Ed. T. Tsuada, Y. Sasaki, R. Kawashima). Academic Press, San Diego, pp. 483-514
  20. Gieseke, D., M. Stangassinger and W. Tiemeyer. 1984. Nucleic acid digestion and urinary purine metabolites in sheep nourished by intragastric infusions. Can. J. Anim. Sci. 64:144-145 https://doi.org/10.4141/cjas84-196
  21. Gonda, H. L. and J. E. Lindberg. 1994. Evaluation of dietary nitrogen utilization in dairy cows based on urea concentrations in blood, urine and milk, and on urinary concentrations of purine derivatives. Acta Agricultae Scandinavia, Section A, Anim. Sci. 44:236-245
  22. Gonda, H. L., M. Emanuelson and M. Murphy. 1996. The effect of roughage to concentrate ratio in the diet on nitrogen and purine metabolism in dairy cows. Anim. Feed. Sci. Technol. 64:27-42 https://doi.org/10.1016/S0377-8401(96)01049-8
  23. Hall, A., M. Blummel, W. Thorpe, F. R. Bidinger and C. T. Hash. 2004. Sorghum and pearl millet as food crops in India. Anim. Nutr. Feed Technol. 4:1-15
  24. IAEA-TECDOC-945. 1997. Estimation of Rumen Microbial Protein Production from Purine Derivatives in Urine. International Atomic Energy Agency, Vienna
  25. Lechner-Doll, M., M. Kaske and W. V. Engelhardt. 1991. Factors affecting the mean retention time of particles in the forestomach of ruminants and camelides. In: Physiological Aspect of Digestion and Metabolism in Ruminants. (Ed. T. Tsuada, Y. Sasaki and R. Kawashima). Academic Press, San Diego, pp. 455-482
  26. Malestein, A., A. T. van't Klooster, G. H. M. Counotte and R. A. Prins. 1981. Concentrate feeding and ruminal fermentation: 1. Influence of the frequency of feeding concentrates on rumen acid composition, feed intake and milk production. Neth. J. Agric. Sci. 29:239-248
  27. Martin Orue, S. M., J. Balcells, J. A. Gauda and M. Fondevila. 2000. Microbial nitrogen production in growing heifers: direct measurement of duodenal flow purine bases versus urinary excretion of purine derivatives as estimation procedures. Anim. Feed Sci. Technol. 88:171-188 https://doi.org/10.1016/S0377-8401(00)00221-2
  28. McAllan, A. B. and R. H. Smith. 1973. Degradation of nucleic acids derivatives by rumen Bacteria in vitro. Br. J. Nutr. 29:467-474 https://doi.org/10.1079/BJN19730122
  29. Moorby, J. M. and R. J. Dewhurst. 1993. Diurnal variation in urinary purine derivative excretion of dairy cows on different feeding patterns. Anim. Prod. 56:464
  30. Mould, F. L. and E. R. Orskov. 1983. Manipulation of rumen fluid pH and its influence on cellulolysis insacco, dry matter degradation and the microflora of sheep offered either hay or concentrate. Anim. Feed Sci. Technol. 10:1-14 https://doi.org/10.1016/0377-8401(83)90002-0
  31. Mould, F. L., E. R. Orskov and S. O. Mann. 1983. Associative effects of mixed feeds: 1. Effects of type and level of supplement and the influence of the minimal pH on cellulolysis in-vivo and dry matter digestion of various roughages. Anim. Feed Sci. Technol. 10:15-30 https://doi.org/10.1016/0377-8401(83)90003-2
  32. Nsahlai, I. V., P. O. Osuji and N. N. Umunna. 2000. Effect of form and of quality of feed on the concentration of purine derivatives in urinary spot samples, daily microbial nitrogen supply and predictability of intake. Anim. Feed Sci. Technol. 85:223-238 https://doi.org/10.1016/S0377-8401(00)00138-3
  33. Puchala, R. and G. W. Kulasek, 1992. Estimation of microbial protein from the rumen of sheep using microbial nucleic acid and urinary excretion of purine derivatives. Can. J. Anim. Sci. 72:821-830 https://doi.org/10.4141/cjas92-094
  34. Puchala, R., J. A. Shelford, W. Barej, G. W. Kulasek, H. Pior, M. V. Keyserlingk and N. Makoni. 1993. Urinary excretion of psuedouridine and purine metabolites in ruminants. J. Anim. Physiol. Anim. Nutr. 69:186-193 https://doi.org/10.1111/j.1439-0396.1993.tb00804.x
  35. Santoso, B., S. Kume, K. Nonaka, Y. Gamo, K. Kimura and J. Takahashi. 2003. Influence of $\beta$ 1-4 galacto-oligosaccharides supplementation on nitrogen utilization, rumen fermentation, and microbial nitrogen supply in dairy cows fed silage. Asian-Aust. J. Anim. Sci. 16:1137-1142 https://doi.org/10.5713/ajas.2003.1137
  36. Schneider, B. H. and W. P. Flatt. 1975. Evaluation of Feeds Through Digestibility Experiments. The University of Georgia Press, Athens, pp. 57-78
  37. Shingfield, K. J. and N. W. Offer. 1998. Evaluation of the spot urine sampling technique to assess urinary purine derivative excretion in lactating dairy cows. Anim. Sci. 66:557-568 https://doi.org/10.1017/S1357729800009139
  38. Smith, R. H. and A. B. Mc Allan. 1970. Nucleic acid metabolism in ruminants: 2. Formation of microbial nucleic acids in the rumen in relation to the digestion of food nitrogen, and the fate of dietary nucleic acids. Br. J. Nutr. 24:545-556 https://doi.org/10.1079/BJN19700052
  39. Snedecor, G. W. and W. C. Cochran. 1994. Statistical methods. 8th edn. Iowa State University, Iowa, USA
  40. Susmel, P., M. Spanghero, B. Stefanon, C. R. Mills and E. Plazzota. 1994. Digestibility and allantoin excretion in cows fed diets differing in nitrogen content. Liv. Prod. Sci. 39:97-99 https://doi.org/10.1016/0301-6226(94)90160-0
  41. Tafaj, M., V. Tafaj, M. V. Kolaneci, B. Junck, A. Maulbetsch, H. Steingass and W. Drochner. 2005. Influence of fibre content and concentrate level on chewing activity, ruminal digestion, digestar passage rate and nutrient digestibility in dairy cows in lactation. Asian-Aust. J. Anim. Sci. 18:1116-1124 https://doi.org/10.5713/ajas.2005.1116
  42. Topps, J. H. and R. C. Elliot. 1965. Relationships between concentrations of ruminal nucleic acid and excretion of purine derivatives by sheep. Nature 205:498-499
  43. Verbic, J., X. B. Chen, N. A. MacLeod and E. R. Orskov. 1990. Excretion of purine derivatives by ruminants: effects of microbial nucleic acids infusion on purine derivative excretion by steers. J. Agric. Sci. (Camb.) 114:243-248 https://doi.org/10.1017/S0021859600072610
  44. Wanapat, M. and O. Pimpa. 1999. Effect of ruminal NH3-N levels on ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes. Asian-Aust. J. Anim. Sci. 12:904-907 https://doi.org/10.5713/ajas.1999.904

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