미생물학회지 (Korean Journal of Microbiology)

  • 제42권3호
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  • Pages.230-234
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  • 2006
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

마우스레트로바이러스에 의한 합포체 형성 기작 분석

Analysis of Syncytium Formation Mechanism induced by Ecotropic Murine Retrovirus

  • 배은혜 (단국대학교 첨단과학대학 미생물학전공 및 기초과학 연구소) ;
  • 박성한 (단국대학교 첨단과학대학 미생물학전공 및 기초과학 연구소) ;
  • 정용태 (단국대학교 첨단과학대학 미생물학전공 및 기초과학 연구소)
  • Bae, Eun-Hye (Department of Microbiology and Institute Basic Science, College of Advanced Science, Dankook University) ;
  • Park, Sung-Han (Department of Microbiology and Institute Basic Science, College of Advanced Science, Dankook University) ;
  • Jung, Yong-Tae (Department of Microbiology and Institute Basic Science, College of Advanced Science, Dankook University)
  • 발행 : 2006.09.30
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초록

레트로바이러스의 합포체(syncytia)형성 기작 연구를 위해 합포체 형성을 유도하는 새로운 ecotropic 마우스레트로바이러스(Friend murine leukemia virus)변이주를 실험에 사용하였다. 마우스레트로바이러스의 외막에 존재하는 당단백질 중 수용체와 결합하는 부위의 아미노산을 변화시키면 합포체를 형성할 수 있음이 이미 밝혀졌다. 본 연구에서는 합포체 유도 마우스레트로바이러스 외막 당단백질을 가진 pseudotype 레트로바이러스 벡터로 부터도 이러한 융합 현상이 일어날 수 있는지 알아보았다. 마우스 세포주인 M. dunni에 pseudotype 바이러스를 감염시킨 결과 레트로바이러스 벡터 매개에 의한 바이러스-세포간 융합 현상은 일어나지 않았다. 이러한 실험결과는 합퐁체 형성이 바이러스 복제가 가능한 합포체 유도 마우스레트로바이러스에만 일어남을 나타낸다. 또한 ecotropic 마우스레트로바이러스 수용체의 농도와 막 융합과의 상관관계도 없는 것으로 밝혀졌다.

To study the mechanism of syncytium formation, novel syncytia-inducing ecotropic murine retrovirus was used. Our previous result showed that amino acid substitutions at the RBD (receptor binding domain) of envelope glycoprotein contribute to syncytium formation. In this study, we have investigated if this fusion phenomenon could occur with retroviral vectors pseudotyped with the novel syncytia-inducing ecotropic murine leukemia virus Env. We have found that these vectors were not able to mediate virus-to-cell fusion in M. dunni murine cell lines. These findings indicate that syncytia-inducing ecotropic murine leukemia virus is capable of generating syncytia during its replication. There was also no correlation between the level of ecotropic murine leukemia virus receptor (mCAT-1) and the fusogenic effect.

키워드

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