Journal of Ecology and Environment

한국생태학회 (The Ecological Society of Korea)
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Changes of Thiols and Oxidative Stress in Tomato Seedlings Exposed to Cadmium

  • Cho, Un-Haing (Department of Biology, Changwon National University) ;
  • Seo, Nam-Ho (Department of Biology, Changwon National University)
  • 발행 : 2006.02.01
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초록

Tomato (Lycopersicon esculentum Mill) seedlings exposed to various concentrations of $CdCl_2(0{\sim}100{\mu}M)$ in a nutrient solution for up to 9 days were analyzed with respect to the thiol changes and oxidative stress. The Cd exposure increased total non-protein thiols (NPT) and cysteine in both leaves and roots, total glutathione in leaves, and the ratios of oxidized glutathione (GSSG)/reduced glutathione (GSH) in both leaves and roots, but decreased the ratio of dehydroascorbate (DASA)/ascorbate(ASA) in leaves. Our results suggest that the Cd-induced GSH depletion due to thiol synthesis and oxidation alters the antioxidant activity of seedlings for $H_2O_2$, and the subsequent $H_2O_2$ accumulationand oxidative stress result in phytotoxicity.

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