DOI QR코드

DOI QR Code

Effects of Calcium and Nitrogen on the Growth and Antioxidative Enzyme Activity in Soybean (Glycine max) under Saline Condition

  • Published : 2006.04.01

Abstract

Growth of G. max treated with $NO_3^-$-N was decreased by high NaCl treatments, but $NH_4NO_3$-fed plants showed good growth with enhanced activity of antioxidative enzymes (SOD and APX). Especially, activity of APX was higher in 5 mM $NH_4NO_3$-fed plants than other types of N-supplied plants throughout the stress period. Higher SOD activity under salt stress was accompanied by increase in APX activity in 5 mM $NH_4NO_3$-fed plants. Similarly, application of calcium confirmed somewhat positive effects on growth. Salt-treated soybean plants showed the best growth response with the increase of SOD and APX activity at an additional 5 mM calcium treatment. Especially, the increase of SOD activity through the strengthened CuZn-SOD isoform was remarkable.

Keywords

References

  1. Alston AM, Graham RD. 1982. The influence of soil nitrogen status and previous crop on nitrogen fixation (acetylene reduction) in barrel medic, Medicago trunculata. Aust J Soil 27: 462-469
  2. Asada K. 1994. Production and action of active oxygen species in photosynthetic tissue. In: Causes of photooxidative stress in plant and amelioration of defense system (Foyer CH, Mullineaux PM, eds), CRC Press, Boca Raton, FL. pp 77-103
  3. Bekki A, Trinchant JC, Rigaud J. 1987. Nitrogen fixation (C2H4 reduction) by Medicago nodules and bacteroids under sodium chloride stress. Plant Physiol 74: 72-76
  4. Beyer WF, Fridovich I. 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161: 559-566 https://doi.org/10.1016/0003-2697(87)90489-1
  5. Casano LM, Lascano HR, Trippi VS. 1994. Hydroxyl radicals and a thylakoid-bound endopeptidase are involved in light and oxygen- induced proteolysis in oat chloroplasts. Plant Cell Physiol 35: 145-152
  6. Charles SA, Halliwell B. 1981. Light activation of fructose bisphosphate in isolated spinach chloroplasts and deactivation by hydrogen peroxide. Planta 151: 242-246 https://doi.org/10.1007/BF00395175
  7. Cramer MD, Lewis OAM. 1993. The influence of $NO_3^-$ and $NH_4^+$ nutrition on the carbon and nitrogen partitioning characteristics of wheat (Triticum aestivum L.) and maize (Zea mays L.) plants. Plant Soil 154: 289-300 https://doi.org/10.1007/BF00012534
  8. Fridovich I. 1986. Superoxide dismutases. Adv Enzymol Relat Areas Mol Biol 58: 61-97
  9. Fridovich I. 1995. Superoxide radical and superoxide dismutase. Annu Rev Biochem 64: 97-112 https://doi.org/10.1146/annurev.bi.64.070195.000525
  10. Hagar H, Ueda N, Shah SV. 1996. Role of reactive oxygen metabolites in DNA damage and cell death in chemical hypoxic injury to LLC-PK1 cells. Amer J Physiol 271: F209-F215
  11. Halliwell B, Gutteridge JMC. 1986. Iron and free radical reactions: two aspects of antioxidant protection. Trends Biochem Sci 11: 372-375 https://doi.org/10.1016/0968-0004(86)90207-0
  12. Hawkins HJ, Lewis OAM. 1993a. Combination effect of NaCl salinity, nitrogen form and calcium concentration on the growth, ionic content and gaseous exchange properties of Triticum aestivum L. cv. Gamtoos. New Phytol 124: 161-170 https://doi.org/10.1111/j.1469-8137.1993.tb03806.x
  13. Hawkins HJ, Lewis OAM. 1993b. Effect of NaCl salinity, nitrogen form, calcium and potassium concentration on nitrogen uptake and kinetics in Triticum aestivum L. cv. Gamtoos. New Phytol 124: 171-177 https://doi.org/10.1111/j.1469-8137.1993.tb03807.x
  14. Hernandez JA, Campillo A, Jimenez A, Alarcon JJ, Sevilla F. 1999. Response of antioxidant systems and leaf water relations to NaCl stress in pea plants. New Phytol 141:241-251 https://doi.org/10.1046/j.1469-8137.1999.00341.x
  15. Jimenez A, Hernandez JA, del Rio LA, Sevilla F. 1997. Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves. Plant Physiol 114: 275-284 https://doi.org/10.1104/pp.114.1.275
  16. Lauter DJ, Munns DN, Clarkin KL. 1981. Salt response of chickpeas influenced by N supply. Agron J 73: 961-966 https://doi.org/10.2134/agronj1981.00021962007300060013x
  17. Lazof D, Lauchli A. 1991. The nutritional status of the apical meristem of Lactuca sativa as affected by NaCl salinization: An electron-probe microbe microanalytic study. Planta 184: 334- 342
  18. Legge RL, Thompson E, Baker JE, Lieberman M. 1982. The effect of calcium on the fluidity and phase properties of microsomal membranes isolated from postclimacteric Gloden Delicious apples. Plant Cell Physiol 23: 161-169
  19. Lewis OAM, Chadwick S. 1983. An $^{15}N$ investigation into nitrogen assimilation in hydroponically-grown barley (Hordeum vulgare L. cv. Clipper) in responses to nitrate, ammonium and mixed nitrate and ammonium nutrition. New Phytol 95: 635-646 https://doi.org/10.1111/j.1469-8137.1983.tb03527.x
  20. Lewis OAM, Leidi EO, Lips SH. 1989. Effect of nitrogen sources on growth response to salinity stress in maize and wheat. New Phytol 111: 155-160 https://doi.org/10.1111/j.1469-8137.1989.tb00676.x
  21. Liochev S, Fridovich I. 1991. Effects of overproduction of superoxide dismutase on the toxicity of paraquat toward Escherichiacoli. J Biol Chem 266: 8747-8750
  22. Lips SH, Leidi EO, Silberbush M, Soares MIM, Lewis OAM. 1990. Physiolgical aspects of ammonium and nitrate fertilization. J Plant Nutr 13: 1271-1289 https://doi.org/10.1080/01904169009364151
  23. Loneragan JF, Snowball K, Simmons WJ. 1968. Response of plants to calcium concentration in solution culture. Aust J Agric Res 19: 845-857 https://doi.org/10.1071/AR9680845
  24. Loneragan JF, Snowball K. 1969. Calcium requirements of plants. Aust J Agric Res 20: 465-478 https://doi.org/10.1071/AR9690465
  25. Lowry OH, Rosenbrough NJ, Farr AL, Randall HJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275
  26. Lynch J, Cramer GR, Lauchli A. 1987. Salinity reduces membraneassociated calcium in corn root protoplasts. J Biol Chem 83: 390-394
  27. Mcknight T. 1949. Efficiency of isolates of Rhizobium in the cow pea group, with proposed additions to this group. Queensland J Agric Sci 6: 61-76
  28. Noctor G, Foyer CH. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Annu Rev Plant Physiol Plnat Mol Biol 49: 249-279 https://doi.org/10.1146/annurev.arplant.49.1.249
  29. Rengel Z. 1992. The role of calcium in salt toxicity. Plant Cell Environ 15: 625-632 https://doi.org/10.1111/j.1365-3040.1992.tb01004.x
  30. Scandalios JG. 1993. Oxygen stress and superoxide dismutase. Plant Physiol 101: 7-12 https://doi.org/10.1104/pp.101.1.7
  31. Scandalios JG. 1997. Molecular genetics of superoxide dismutase in plants. In: Oxidative stress and the molecular biology of antioxidant defenses (Scandalios JG, eds). Cold Spring Harbor Laboratory Press, New York, pp 527-568
  32. Scott M, Meshnick S, Eaton J. 1987. Superoxide dismutase rich bacteria: paradoxial increase in oxidant toxicity. J Biol Chem 262: 3640-3645
  33. Shalata A, Tal M. 1998. The effect of salt stress on lipid peroxidation and antioxidants in the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Plant Physiol 104: 169-174 https://doi.org/10.1034/j.1399-3054.1998.1040204.x
  34. Singleton PW, Bohool B. 1984. Effect of salinity on nodule formation by soybean. Can J Plant Sci 61: 231-239 https://doi.org/10.4141/cjps81-035
  35. Srivastava HS, Singh RP. 1999. Nitrogen nutrition and plant growth. Science Pub. Enfield, New Hampshire
  36. Tu JC. 1981. Effect of salinity on Rhizobium-root hair interaction, nodulation and growth of soybean. Can J Plant Sci 61: 231-239 https://doi.org/10.4141/cjps81-035
  37. Yeo AR, Kramer D, Lauchli A. 1977. Ion distribution in salt-stressed mature Zea mays root in relation ultrastructure and retention of sodium. J Exp Bot 28: 17-29 https://doi.org/10.1093/jxb/28.1.17
  38. Yousef AN, Sprent JL. 1983. Effect of NaCl on growth, nitrogen incorporation and chemical composition of inoculated and $NH_4NO_3$ fertilized Vicia faba L. plant. J Exp Bot 34: 941-950 https://doi.org/10.1093/jxb/34.8.941
  39. Zhu JK. 2000. Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physiol 124: 941-948 https://doi.org/10.1104/pp.124.3.941

Cited by

  1. Nitrogen nutrition and adaptation of glycophytes to saline environment: a review vol.64, pp.9, 2018, https://doi.org/10.1080/03650340.2017.1419571