Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Antioxidant-Producing Alteromonas macleodii HJ-14 Isolated from Seawater

  • Yeo Soo-Hwan (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Kim Hyun-Jin (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Yoon Jung-Hoon (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim Hyun-Soo (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Hwang Yong-Il (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Lee Seung-Cheol (Division of Food Science and Biotechnology, Kyungnam University)
  • Published : 2006.05.01
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Abstract

A bacterial strain HJ-14 was isolated as a producer of antioxidants from the coast of Jinhae in Korea. The isolate showed 43.4mol% of G+C content, and contained dihydrogenated ubiquinone with Q8 as a major quinone. Chemotaxonomic analysis as well as phylogenetic analysis, based on the 16S rDNA sequence, identified the isolate as a member of Alteromonas macleodii. For antioxidant production, the optimum medium composition was determined to be 3% dextrin, 0.5% ammonium sulfate, and 2-6% sodium chloride. Optimum culture conditions for production of antioxidant materials with strain HJ-14 were at pH 6.0-8.0 and $25-37^{\circ}C$. The chloroform extract of strain HJ-14 broth showed 1.96-17.5-fold higher antioxidant activity than other organic solvents in term of electron donating ability.

Keywords

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