The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)

The Korean Academy of Prosthodonitics (대한치과보철학회)
권호 표지

THE EFFECT OF FRUCTOSE ON THE METABOLISM OF SUCROSE BY STREPTOCOCCUS MUTANS

Streptococcus mutans의 자당 대사에 미치는 과당의 영향

  • Shim Jig-Hyeon (Department of Prosthodontics, College of Dentistry, Chonnam National University) ;
  • Vang Mong-Sook (Department of Prosthodontics, College of Dentistry, Chonnam National University) ;
  • Yang Hong-So (Department of Prosthodontics, College of Dentistry, Chonnam National University) ;
  • Park Sang-Won (Department of Prosthodontics, College of Dentistry, Chonnam National University) ;
  • Park Ha-Ok (Department of Prosthodontics, College of Dentistry, Chonnam National University) ;
  • Oh Jong-Suk (Department of Microbiology, College of Medicine, Chonnam National University) ;
  • Lee Jai-Bong (Department of Prosthodontics, Graduate School, Seoul National University)
  • 심직현 (전남대학교 치과대학 보철학교실) ;
  • 방몽숙 (전남대학교 치과대학 보철학교실) ;
  • 양홍서 (전남대학교 치과대학 보철학교실) ;
  • 박상원 (전남대학교 치과대학 보철학교실) ;
  • 박하옥 (전남대학교 치과대학 보철학교실) ;
  • 오종석 (전남대학교 의과대학 미생물학교실) ;
  • 이재봉 (서울대학교 치과대학 보철학교실)
  • Published : 2006.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Statement of problem: Streptococcus produces energy and forms extracellular polysaccharides by metabolizing sucrose. Insoluble glucan, a kind of extracellular polysaccharide, is the important material of dental plaque. Fructose affects the metabolism of sucrose. Purpose: The purpose of this study was to evaluate the effect of fructose on the metabolism of sucrose in Streptococcus mutans. Materials and methods: To determine the effect of fructose on the formation of artificial plaque by Streptococcus mutans Ingbritt, S. mutans and fructose were placed in beakers containing M17 broth and sucrose. The wires were hung on frameworks inserted into cork stoppers, and then immersed in each of the beakers. After the incubation with gentle shaking, each wire was weighed. To analyze the effect of fructose on the sucrose metabolism by S. mutans or glucosyltransferase, S. mutans and fructose were placed in M17 broth containing sucrose. After the incubation. the remaining sucrose and polymers were analysed by thin layer chromatography. Results: The following results were obtained; 1. When Streptococcus mutans was cultured in the media containing 3% sucrose for 8 hours, the mean weight of formed artificial plaque on the wires was $124.3{\pm}3.0mg$, whereas being reduced to $20.7{\pm}10.2mg$ in the media added with 3% sucrose and 4% fructose(p<0.05) 2. When the control containing glucose was added with sucrose, the optical density of Streptococcus mutans solution cultured for 24 hours was not increased compared with the control, while being increased by adding with fructose. 3. When Streptococcus mutans was incubated in the media added with sucrose and fructose for 8 hours, the number of viable cells was increased compared with the media added with sucrose. 4. The amount of remained sucrose was increased in Streptococcus mutans culture supernatant of media added with sucrose and fructose than with sucrose only. but the amount of produced insoluble glucan was decreased. 5. The amounts of remained sucrose and produced soluble glucan were increased in the culture of glucosyltransferase-contained media added with sucrose and fructose than with sucrose only, but the amount of produced insoluble glucan was decreased . Conclusion: These results indicated that the sucrose metabolism and the production of insoluble glucan were inhibited in Streptococcus mutans by adding fructose in the media containing sucrose.

Keywords

References

  1. Park KC. Tooth plaque (2). Dental research 1998:43:23-30
  2. Clarke JK. On the bacterial factor in the aetiology of dental caries. Br J Exp Pathol 1924;5:141-147
  3. Hamada S. Slade HD. Biology. immunology and cariogenicity of Streptococcus mutans. Microbiol Rev 1980:44:331-384
  4. Rolla G. Scheie AA Ciardi JE. Role of sucrose in plaque formation. Scand. J Dent Res 1985: 93: 105-111
  5. Guggenheim B. Extracellular polysaccharides and microbial plaque. Int Dent J 1970:20:657-678
  6. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986; 50: 353-380
  7. Tanzer JM. Microbiology of dental caries. In: Contemporary oral microbiology and immunology. edited by Slots J. and Taubman M. St. Louis: Mosby, pp. 1992 p.377-424
  8. Halhoul MN, Kleinberg I. Differential determination of glucose and fructose, and glucose- and fructose-yielding substances with anthrone. Ana Biochem 1972;50:337-343 https://doi.org/10.1016/0003-2697(72)90042-5
  9. McDougall WA. Studies on the dental plaque. I. The histology of the dental plaque and its attachment. Aust Dent J 1963:8:261-273 https://doi.org/10.1111/j.1834-7819.1963.tb03253.x
  10. Newbrun E. Polysaccharide synthesis in plaque. Microbiology Abstract Suppl. Microbial aspect of dental caries III. 1979 p.659
  11. Toda Y. Moro I. Koga T. Asakawa H. Hamada S. Ultrastruture of extracellular polysaccharides produced by serotype c Streptococcus mutans. J Dent Res 1987: 66:1364-1369 https://doi.org/10.1177/00220345870660081601
  12. Gibbons RJ. Banghart SS. Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque. Arch Oral Biol 1967: 12: 11-24 https://doi.org/10.1016/0003-9969(67)90137-9
  13. Dewar MG. Walker GJ. Metabolism of the polysaccharides of human dental plaque. Caries Res 1975:9:21-35 https://doi.org/10.1159/000260139
  14. Gibbons RJ. van Houte J. Bacterial adherence in oral microbial ecology. Ann Rev Microbiol 1975: 29: 19-44 https://doi.org/10.1146/annurev.mi.29.100175.000315
  15. Hammond BF. Dextran production by a human oral strain of Lactobacillus casei. Archs Oral Biol 1969: 14:879-890 https://doi.org/10.1016/0003-9969(69)90266-0
  16. Aoki H. Shiroza T, Hayakawa M, Sato S, Kuramitsu HK. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect Immun 1986:53: 587-594
  17. Hanada N, Kuramitsu HK. Isolation and characterization of the Streptococcus rnutans gtfC gene, coding for synthesis of both soluble and insoluble glucans. Infect Immun 1988:56: 1999-2005
  18. Hanada N, Kuramitsu HK. Isolation and characterization of the Streptococcus mutans gtfC gene, coding for primer-dependent soluble glucan synthesis. Infect Immun 1989:57:2079-2085
  19. Nakano YJ, Kuramitsu HK. Mechanism of Streptococcus mutans glucosyltransferase: hybrid-enzyme analysis. J Bacteriol 1992: 174:5639-5646 https://doi.org/10.1128/jb.174.17.5639-5646.1992