Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

THE EFFECT OF HUMAN DBM($GRAFTON^{(R)}$) GRAFT ON SKULL DEFECT IN THE RABBIT

가토의 두개골 결손부에 이식한 human DBM ($Grafton^{(R)}$)의 효과

  • Kim, Jin-Wook (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Park, In-Suk (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Lee, Sang-Han (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Kim, Chin-Soo (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Jang, Hyun-Jung (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Kwon, Tae-Geon (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Kim, Hyun-Soo (Dept. of Dentistry, Gumi Cha Hospital)
  • 김진욱 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 박인숙 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 이상한 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 김진수 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 장현중 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 권대근 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 김현수 (포천중문의대 구미차병원 치과학교실)
  • Published : 2006.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In oral and maxillofacial surgery, bone graft is very important procedure for functional and esthetic reconstruction. So, many researcher studied about bone graft material like autogenous bone, allograft bone and artificial bone materials. The purpose of this study is to evaluate the quantity of bone generation induced by $Grafton^{(R)}$ graft, human allogenic demineralized bone matrix. Total 24 sites of artificial bony defects prepared using trephin bur(diameter 8 mm) on parietal bone of six adult New Zealand White rabbits. Experimental group had six defect sites which grafted $Grafton^{(R)}$(0.1 cc). Active control group had nine defect sites, into which fresh autogenous bone harvested from own parietal bone was grafted and passive control group had nine defect sites without bone graft. After six weeks postoperatively, the rabbits were sacrificed. The defects and surrounding tissue were harvested and decalcified in 10% EDTA, 10% foamic-acid. Specimens were stained with H&E. New bone area percentage in whole defect area was measured by IMT(VT) image analysis program. Quantity of bone by $Grafton^{(R)}$ graft was smaller than that of autograft and larger than that of empty defects. In histologic view $Grafton^{(R)}$ graft site and autograft site showed similar healing progress but it was observed that newly formed bone in active control group was more mature. In empty defect, quantity and thickness of new bone formation was smaller than in $Grafton^{(R)}$-grafted defect. $Grafton^{(R)}$ is supposed to be a useful bone graft material instead of autogenous bone if proper maintenance for graft material stability and enough healing time were obtained.

Keywords

References

  1. Sung-Hoon Lee, Seung-Il Song, Ji-Young Han et al : The experimental study of the bone regeneration on $\beta$-TCO in rabbit cranial bone. J Kor Oral Maxillofac Surg 30 : 282, 2004
  2. Hopp SG, Dahners LE, Gilbert JA : A study of the mechanical strenth of long bone defects treated with various bone autograft substitutes: an experimental investigation in the rabbit. J Orthop Res 7(4) : 579, 1989 https://doi.org/10.1002/jor.1100070416
  3. Goldberg VM, Stevenson S : Nature history of autograft and allograft. Clin Orthop 225 : 7, 1987
  4. Fernyhough JC, Schimandle JH, Weigel MC, et al : Chronic donor site pain complicating bone graft harvesting from the posterior iliac crest for spinal fusion. Spine 17 : 1474, 1992 https://doi.org/10.1097/00007632-199212000-00006
  5. Arrington ED, Smith WJ, Chambers HG, et al : Complications of iliac crest bone graft harvesting. Clin Orthop 329 : 300, 1996 https://doi.org/10.1097/00003086-199608000-00037
  6. Giovanni Cricchio, Stefan Lundgren : Donor site morbidity in two different approaches to anterior iliac crest bone harvesting. Clin Implnat Dentistry and related research 5(3) : 161, 2003 https://doi.org/10.1111/j.1708-8208.2003.tb00198.x
  7. Sun-Yuo Ryu, Il-Kyu Kim, Jong-Chull Kim et al : Reconstruction of mandibular bone defect with autogenous iliac bone graft. J Kor Oral Maxillofac Surg 12 : 65, 1986
  8. Robert AR : Prospective evaluation of morbidity associated with iliac crest harvest for aloveolar cleft grafting. J Oral Maxillofac Surg 55 : 219, 1997 https://doi.org/10.1016/S0278-2391(97)90528-0
  9. John LU, Hideki Murakami, Kim HS et al : Evidence of osteoinduction by Grafton demineralized bone matrix in nonhuman primate spinal fusion. Spine 29 : 360, 2004 https://doi.org/10.1097/01.BRS.0000090823.12652.F9
  10. George J. Martin, Jr, Scott D. Boden, Louisa Titus et al : New formulations of demineralized bone matrix as a more effective graft alternative in experimental posterolateral lumbar spine arthrodesis. Spine 24(7) : 637, 1999 https://doi.org/10.1097/00007632-199904010-00005
  11. S. Cheung, K. Westerheide, B. Ziran : Efficacy of contained metaphyseal and periarticular defects treated with two different demineralized bone matrix allografts. Int Orthop 27(1) : 56, 2003
  12. Frank P. Cammisa, Jr, Gary Lowery, Steven R. Garfin et al : Two-year fusion rate equivalency between $Grafton^{circledR}$ DBM Gel and autograft in posterolateral spine fusion. Spine 29(6) : 660, 2004 https://doi.org/10.1097/01.BRS.0000116588.17129.B9
  13. Yu-Po Lee, Mark Jo, Mario Luna et al : The efficacy of different commercially available demineralized bone matrix substances in an athymic rat model. J Spinal Disord Tech 18(5) : 439, 2005 https://doi.org/10.1097/01.bsd.0000175696.66049.f7
  14. A. Bark M. Rabie, Mei Lu : Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor durin healing of allogeneic bone graft. Arch Oral Biol 49 : 1025, 2005 https://doi.org/10.1016/j.archoralbio.2004.05.012
  15. Cameron M. L. Clokie, Hassan Moghadam, Micheal T. jackson et al : Closure of critical sized defects with allogenic and alloplastic bone substitutes. J Craniofac Surg 13(1) : 111, 2002 https://doi.org/10.1097/00001665-200201000-00026
  16. DeLuca L, Raszewski R, Tresser N et al : The fate of preserved autogenous bone graft. Plast Reconstr Surg 99(5) : 1324, 1997 https://doi.org/10.1097/00006534-199704001-00019
  17. Hassan G, George K.B, Howard H.I et al : Histomorphometric evaluation of bone regeneration using allogeneic and alloplastic bone substitutes. J Oral Maxillofac Surg 62(2) : 202, 2004 https://doi.org/10.1016/j.joms.2003.10.002
  18. William JB, Irvine Jw : Preparation of the inorganic matrix of bone. Science 119 : 771, 1954 https://doi.org/10.1126/science.119.3100.771
  19. Oikarinen J, Korhonen LK : The bone inductive capacity of various bone transplanting materials used for treatment of experimental bone defect. Clin Orthop Relat Res 140 : 208, 1979
  20. Sullivan WG, Szwajkun PR : Revascularization of cranial versus iliac crest bone grafts in the rat. Plast reconstr Surg 87 : 1105, 1991 https://doi.org/10.1097/00006534-199106000-00013
  21. Urist MR : Bone: formation by autoinduction. Science 150 : 893, 1965 https://doi.org/10.1126/science.150.3698.893
  22. Boden SD, Zdeblick TA, Sandhu HS, et al : The use of rhBMP-2 in interbody fusion cages: Definitive evidence of osteoinduction in humans: A preliminary report. Spine 25 : 376, 2000 https://doi.org/10.1097/00007632-200002010-00020
  23. Guizzardi S, Di Sliverstri M, Scandroglio R, et al : Implants of heterologous demineralized bone matrix for induction of posterior spinal fusion in rats. Spine 17 : 701, 1992 https://doi.org/10.1097/00007632-199206000-00010
  24. Satoshi Takikawa : Comparative evaluation of the osteoinductivity of two formulations of human demineralized bone matrix. J Biomed Mater Res 65A : 37, 2003 https://doi.org/10.1002/jbm.a.10345
  25. Syftestad GT, Urist MR : Bone age. Clin Orthop 162 : 288, 1982
  26. Schwartz Z, Somers A, Mellonig JT, et al : Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation is depend on donor age but not gender. J Periodontol 69 : 470, 1998 https://doi.org/10.1902/jop.1998.69.4.470
  27. Nyssen-Behets C, Delaere O, Duchesne PY et al A : Aging effect on inductive capacity of human demineralized bone matrix. Arch Orthop Trauma Surg 115 : 303, 1996 https://doi.org/10.1007/BF00420320
  28. Nishimoto SK, Chang CH, Gendler E et al : The effect of aging on bone formation in rats: Biochemical and histological evidence for decreased bone formation capacity. Calcif Tissue Int 37 : 617, 1985 https://doi.org/10.1007/BF02554919
  29. Jong-cheoll Jeong, Sun-Youl Ryu : A study on the regenerative capacity of endochondral and membranous demineralized allogenic bone matrix graft in rabbit. J Kor Oral Maxillofac Surg 20 : 413, 1994