YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Alteration of Cytosolic Ca$^{2+}$ Signal by Cryopreservation in Pig Sperm

동결 보존에 의한 돼지 정자 세포질 칼슘 신호의 변화

  • Lee, Sun-Woo (College of Pharmacy, Chungnam National University) ;
  • Li, Yu-Hua (College of Pharmacy, Chungnam National University) ;
  • Kim, Joon-Chul (College of Pharmacy, Chungnam National University) ;
  • Myung, Pyung-Keun (College of Pharmacy, Chungnam National University) ;
  • Park, Chang-Sik (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Woo, Sun-Hee (College of Pharmacy, Chungnam National University)
  • 이선우 (충남대학교 약학대학) ;
  • 이옥화 (충남대학교 약학대학) ;
  • 김준철 (충남대학교 약학대학) ;
  • 명평근 (충남대학교 약학대학) ;
  • 박창식 (충남대학교 동물자원학부 형질전환 복제돼지 연구센터) ;
  • 우선희 (충남대학교 약학대학)
  • Published : 2006.12.31
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Abstract

Although mammalian sperms are cryopreserved for in vitro fertilization a process of cryopreservation decreases the fertility. Acrosome reaction requires depolarization-induced Ca$^{2+}$ influx and Ca$^{2+}$ releases from the Ca$^{2+}$ stores. To examine whether the cellular Ca$^{2+}$ mobilization is altered by a sperm cryopreservation we compared cytosolic Ca$^{2+}$ signals between fresh and cryopreserved pig sperms using confocal Ca$^{2+}$ imaging. The magnitudes of depolarization induced Ca$^{2+}$ increases were significantly smaller in cryopreserved sperms. Exposures to 10 mM caffeine or 5 ${\mu}$M thapsigargin elicited less Ca$^{2+}$ increases in the cryopreserved sperms compared to fresh sperms. In addition, progesterone-trig-gered Ca$^{2+}$ rises, that are thought to enhance acrosome reaction, were completely abolished in the cryopreserved sperms. These results suggest that storage and(/or) release of Ca$^{2+}$ from the intracellular Ca$^{2+}$ stores in pig sperms are significantly impaired by the process of cryopreservation.

Keywords

References

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