Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Purification of Total Ginsesides with Macroporous Resins and Their Biological Activities

  • Li, Huayue (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Jin, Haizhu (Department of Biochemistry, Yantai University) ;
  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • Published : 2006.10.25
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Abstract

Total ginsenosides were purified and their antioxidant, antibacterial and anticancer activities were measured. The crude extracts of ginseng, which were extracted with 75% ethanol by ultrasonification method, were firstly purified on AB-8 macroporous adsorption column to remove water soluble impurities, and decolored on Amberlite IRA 900 Cl anion-exchange column. Then, they were purified on Amberlite XAD16 adsorption column to delete the non-polar impurities. Total ginsenosides contents of the purified extracts were 79.4%, 71.7% and 72.5% in cultured wild ginseng, red ginseng and white ginseng, which were significantly increased than those of crude extracts. All of the three extracts showed concentration-dependant scavenging activities against DPPH radicals, among which white ginseng showed the most powerful activity. Cultured wild ginseng roots showed strongest effect against both B. subtilis PM 125(Gram-positive) and E. coli D31 (Gram-negative) bacteria, while red ginseng and white ginseng only showed the activity against B. subtilis. According to the result of the MTT assay, ail of the three extracts inhibited the growth of U-937 human hohistiocytic lympma cell, which were significantly different (p < 0.05) when compared to the control.

Keywords

References

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