Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Mechanisms of Siegesbeckia Glabrescens-induced Smooth Muscle Cell Apoptosis: Role of iNOS and PKC${\alpha}$

희첨의 iNOS 발현과 PKC${\alpha}$ 억제를 통한 혈관평활근세포의 apoptosis 유도

  • Lee, Seung-Yeul (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Jun, Soo-Young (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Kim, Jong-Bong (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Jang, Hyo-Oil (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Kim, Gil-Whon (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
  • 이승렬 (동국대학교 한의과대학 생리학교실) ;
  • 전수영 (동국대학교 한의과대학 생리학교실) ;
  • 김종봉 (동국대학교 한의과대학 생리학교실) ;
  • 장효일 (동국대학교 한의과대학 생리학교실) ;
  • 김길훤 (동국대학교 한의과대학 생리학교실) ;
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Published : 2006.10.25
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Abstract

We have recently demonstrated that Siegesbeckia glabrescens(SG), a herbal medicine, induces apoptosis via nitric oxide(NO) production in human aortic smooth muscle cells(HASMCS). However, the molecular pathways involved in SG-mediated apoptosis are not fully understand. In the present study, we investigated the cellular mechanisms of SG-induced apoptosis in HASMCS. SG induced NO production through inducible nitric oxide synthase(iNOS) induction. The apoptotic effect of SG was attenuated by L-NNA, a NOS inhibitor. In the presence of L-NNA, the degradation of procaspase-3 by SG was inhibited. SG treatment induced a decrease in Bcl-2 expression but did not affect the expression of Bax. In addition, SG treatment evoked both down-regulation of PKC ${\alpha}$ and inhibition of PKC ${\alpha}$ phosphorylation. These downregulations were reversed by addition of L-NNA. It seems likely to De a downregulation of PKC${\alpha}$ due to long term treatment with PMA. Taken together, these results suggest that apoptotic effects of SG may be due to NO production via iNOS mRNA expression. Furthermore, Bcl-2 and PKC${\alpha}$ downregulation, and caspase-3 activation may be involved in the mechanisms for apoptotic effects by SG.

Keywords

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